Establishment and Preliminary Evaluation of Hepatitis B Virus Transfection Mouse Model by Using Hydrodynamic Injection

doi:10.3969/j.issn.1674-5817.2015.01.001

Abstract

Abstract: Objective To construct a hepatitrs B Virus (HBV) transfection model in C57BL/6 mice with hydrodynamic injection and to further confirm the effectiveness of this mouse model for anti-HBV drug evaluation. Methods C57BL/6 mice were injected hydrodynamically via the tail vein with 10 μg of pAAV-HBV1.2 plasmid in 2 mL PBS. Results The serum level of HBsAg and HBV DNA level were monitored every week. Two weeks after the hydrodynamically injection (HI), all the mice were HBsAg positive while 22.2 % of the mice were still positive five weeks after HI. HBV DNA levels in the serum persisted higher than 10³ copies/ml for four weeks after HI. Histopathological analysis showed inflammatory infiltration and high expression of HBcAg and HBsAg in the transfected mouse liver tissue. In addition, Lamividine were applied for the evaluating the potential of this mouse model for anti-HBV drug evaluation, and data showed significant down regulation of HBV DNA level in the Lamividine treated mice. Conclusion This HBV transfection mouse model maybe effective in screening efficient anti-virus drugs targeting HBV polymerase and in evaluating HBV therapeutic vaccines, and thus promoting the development of novel treatment strategies against chronic hepatitis B infection.

Key words: Hydrodynamic injection, Hepatitis B Virus(HBV), Transfection mouse model, Anti-virus drug

CLC Number:

Q95-33

PENG Xiu-hua, CHEN Li-xiang, REN Xiao-nan, SHI Bi-sheng, XU Chun-hua, ZHOU Wen-jiang, ZHOU Xiao-hui. Establishment and Preliminary Evaluation of Hepatitis B Virus Transfection Mouse Model by Using Hydrodynamic Injection[J]. Laboratory Animal and Comparative Medicine, 2015, 35(1): 1-5.

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