Effects of Hypoxia on Proliferation of Hippocampal Neural Stem Cells in Rat

doi:10.3969/j.issn.1674-5817.2013.02.008

Abstract

Abstract: Objective To observe the effects of hypoxia on proliferation of rat’s hippocampal neural stem cells. Methods The hippocampal neural stem cells from newborn SD rats were cultured in the concentration of 1%, 4%, and 20% oxygen respectively, and the diameter and number of neurospheres were observed under the low oxygen. Results (1) Effects of hypoxia on proliferation efficiency. The number of neurospheres of 1% group was significantly lower than control group, (P<0.05). The number of neurospheres of 4% group was higher than control group, but no significantly difference. (2) Effects of hypoxia on proliferation rate. The diameter of 1% group was significantly shorter than control group at 24 h, 36 h and 48h,and reached the extremely significant levelat 48 h (P<0.01). The diameter of 4% group was longer than control group in 24 h, 36 h and 48 h. There was a significant difference at 36h and an extremely significant difference at48 h. (P<0.01). Conclusion 4% oxygen concentration can not only improve proliferation efficiency of rat’s hippocampal neural stem cells, but also improve its proliferation rate. 1% oxygen concentration can not only inhibit proliferation efficiency of rat’s hippocampal neural stem cells, but also inhibit its proliferation rate. This suggests us that moderate hypoxia can improve the proliferation of rat’s hippocampal neural stem cells, while server hypoxia inhibits its proliferation.

Key words: Hypoxia, Neural stem cells, Proliferation, Hippocampus.

CLC Number:

G804.7Q95-33

WANG Shi-qiang, LOU Shu-jie. Effects of Hypoxia on Proliferation of Hippocampal Neural Stem Cells in Rat[J]. Laboratory Animal and Comparative Medicine, 2013, 33(2): 121-125.

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