Gab2 in Relation to Abnormal Proliferation and Activation in Mice Mast Cell and Organ Impairment Induced by Activating Mutant SHP-2 Tyrosine Phosphatase

doi:10.3969/j.issn.1674-5817.2013.05.001

Abstract

Abstract: Objective To investigate the roles of adaptor protein Gab2 in mast cell abnormal proliferation, activation, and organ damage in SHP-2^D61G/+ mutant mice. Method Four kinds of mouse model genotyped as SHP-2^+/+, Gab2^-/-, SHP-2^D61G/+, and SHP-2^D61G/+/Gab2^-/- were generated from crossbreeding of Gab2^-/- and SHP-2^D61G/+ mouse. Mouse mast cell was generated from bone marrow cell induced by IL-3, and the proliferation of different genotype mast cells stimulated with cytokine was analyzed by MTS assay; Inflammatory cytokines levels in mouse serum and mast cell secretion were measured by ELISA, and alanine aminotransferase (ALT) and cardiac troponin I (cTnI) levels in serum was determined by radioimmunoassay; leukocyte infiltration in mouse tissues and organ impairment were detected with histopathologic detection. Results The Results showed that Gab2 knockout reduced the mice mast cell abnormal proliferation and activation induced by SHP-2^D61G/+ mutation, decreased cytokines secretion accordingly of SHP-2^D61G/+ mice , as well as reduced heart and liver impairment. Conclusion Gab2 knockout weakens the mice mast cell abnormal proliferation and activation induced by SHP-2^D61G/+ mutation, and mitigates the heart and liver damage.

Key words: SHP-2, Gab2, Mast cell abnormal proliferation and activation, Organ impairment

CLC Number:

R363 Q95-33

CHEN Ji, CHEN Zhuo, WANG Xin-yi, ZHENG Hong, QU Cheng-kui, WANG Si-ying. Gab2 in Relation to Abnormal Proliferation and Activation in Mice Mast Cell and Organ Impairment Induced by Activating Mutant SHP-2 Tyrosine Phosphatase[J]. Laboratory Animal and Comparative Medicine, 2013, 33(5): 329-333.

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