Thyroid Function of Chronic Intermittent Hypoxia Rat Model

doi:10.12300/j.issn.1674-5817.2020.153

Abstract

Abstract: Objective To establish a chronic intermittent hypoxia (CIH) rat model to explore the correlation of obstructive sleep apnea-hypopnea syndrome (OSAHS) with thyroid function and its mechanism. Methods SD rats were divided into control group, CIH group, and reoxygenation group (RH group). The CIH animal model was established using a hypoxic chamber and was identified. The expression levels of thyroid stimulating hormone (TRH), thyroid stimulating hormone (TSH), triiodothyronine (T3), and thyroxine (T4) in the serum of each group of rats were detected by radioimmunoassay. The pathological changes in the thyroid tissue in each group were observed by hematoxylin and eosin (HE) staining and toluidine blue staining. Changes in the ultrastructure of the thyroid tissue in rats were observed by electron microscopy. Results The CIH and RH rats had blood oxygen saturations of 70%-92%, and arterial oxygen partial pressures of 60.7-80.1 mmHg, which were close to the pathophysiology of OSAHS, therefore, the CIH animal model was successfully established. Compared with the expression levels of TRH, TSH, T3, and T4 in the serum of rats in the control group, those of rats in the CIH group were significantly reduced (all P < 0.05), while the thyroid mass was significantly increased (P < 0.01). Compared with the expression levels of TRH, TSH, T3, and T4 in the serum of the CIH group rats, those in the serum of the RH group rats increased significantly (all P < 0.05), while the thyroid mass was decreased (P < 0.05). The HE and toluidine blue staining results showed that the thyroid tissue of the control group had intact thyroid follicles, whereas the thyroid tissue of the CIH group showed normal follicular structure loss, some follicular cells with vacuoles, and a dark basal nucleus. The thyroid tissue of the RH group showed normal follicular structure, but some follicular cells were still vacuolar with dark nuclei. Electron microscopy showed that the thyroid tissue of the control group rats had normal follicular cells, while the follicular cells in the thyroid tissue of the CIH group showed nuclear contraction, increased heterochromatin, and matrix loss. The thyroid tissue of the RH group had complete follicular cell structure. Conclusion CIH can cause thyroid dysregulation in rats, and thyroid function partially returns to normal once hypoxia is resolved.

Key words: Obstructive sleep apnea-hypopnea syndrome, Chronic intermittent hypoxia, Thyroid function, Rats

CLC Number:

Q95-33
R-332

TIAN Hui, GAO Long, WANG Zehui. Thyroid Function of Chronic Intermittent Hypoxia Rat Model[J]. Laboratory Animal and Comparative Medicine, 2021, 41(3): 232-237.

Figures/Tables 4

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