Experimental Study on Proliferation, Apoptosis and Migration of Eyelid Fibroblasts in B6-Co Mice

doi:10.3969/j.issn.1674-5817.2019.03.007

Abstract

Abstract: Objective To explore the mechanism of eyelid incomplete closure at birth in C57BL/6J-corneal opacity (B6-Co) mice. Methods The eyelid fibroblasts of B6 and B6-Co mice were isolated, cultured and identified. CCK8 method was used to detect the proliferation of eyelid fibroblasts in B6 and B6-Co mice. Apoptosis of eyelid fibroblasts in B6 and B6-Co mice was detected by Annexin V-FITC. Transwell was used to detect the migration ability of eyelid fibroblasts in B6 and B6-Co mice. Results The eyelid fibroblasts of B6 and B6-Co mice were successfully cultured. The proliferation and migration of eyelid fibroblasts from B6-Co mice were significantly lower than those of B6 mice(P<0.01). The apoptosis of eyelid fibroblasts in B6-Co mice was significantly higher than that of B6 mice (P<0.01). Conclusion The proliferative capacity, apoptosis level and migration ability of eyelid fibroblasts in B6-Co mice were significantly different from those of B6 mice.

Key words: B6-Co mouse, Fibroblast, Cell proliferation, Cell apoptosis, Cell migration

CLC Number:

Q95-33

JING Jin, ZHANG Hai-jun, DU Li-li, JI Gui-qing, SHAO Yi-xiang. Experimental Study on Proliferation, Apoptosis and Migration of Eyelid Fibroblasts in B6-Co Mice[J]. Laboratory Animal and Comparative Medicine, 2019, 39(3): 213-219.

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