A Melanoma Zebrafish Model for Real-time Imaging in vivo

doi:10.3969/j.issn.1674-5817.2018.01.004

Abstract

Abstract: Objective To establish a zebrafish model for real-time monotoring the development of melanoma in vivo and screening relative drugs.Methods The expression effect of pMITFa1-RedV12/hRas plasmid,in which the melanoma was labeled by red fluorescent protein (RFP),was verified in mouse B16 melanocytes.After microinjecting pMITFa1-RedV12/hRas into 1- cell stage of fertilized eggs of zebrafish,we monitored the process of melanoma formation with in vivo imaging system in zebrafish.Results pMITFa1-RedV12/hRas was successfully expressed in mouse melanoma B16 cells.Three days after microinjection of pMITFa1-RedV12/hRas vector to 1-cell satge of zebrafish,melanomas labeled by RFP protein was observed under the fluorescence microscope.Eight weeks later,melanomas were observed clearly by eyes.The rate of tumor formation was 100%.When microinjected into pMITFa1-RedV12/hRas vector into fertilized eggs of TG(zlyz:EGFP) transgenic zebrafish,the interaction between the melanoma cells labeled by RFP protein and the macrophages labeled by green fluorescent protein was clearly observed in in vivo imaging system.Conclusion Successfully established a zebrafish model for real-time monitoring melanoma in vivo,which is cheap and easy to operate.

Key words: Melanoma model, Zebrafish, Real-time imaging

CLC Number:

Q95-33

ZHOU Bin, SHENG Zhe-jin, FENG Chen-zhuo, LI Li-mei. A Melanoma Zebrafish Model for Real-time Imaging in vivo[J]. Laboratory Animal and Comparative Medicine, 2018, 38(1): 22-28.

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