Effects of Proanthocyanin on Expression of IL-1, TGF-β1 and NF-Kappa B Proteins in Rats with Radiation-induced Heart Disease

doi:10.3969/j.issn.1674-5817.2019.05.008

Abstract

Abstract: Objective To observe the effects of proanthocyanin (OPC) on the expression of interleukin-1(IL-1), transformation growth factor-β1(TGF-β1) and nuclear factor-Kappa B(NF-κB) proteins in rats with radiation-induced heart disease (RIHD). To explore the possible role and mechanism of OPC in rats RIHD. Methods Sixty male Sprague-Dawley rats were randomly divided into 6 groups, 10 rats in each group, which were normal control group, the OPC low-dose (50 mg/kg) group, medium-dose (100 mg/kg) group, and large dose were used in the irradiation alone group, (150 mg/kg) group, blueberry (200 mg/kg) group. Except the normal control group, the rats in each group were given a single angi of 6 MV X-rays at a dose of 20 Gy. Rats in the OPC group and the blueberry group were given the corresponding drugs, and the pathological changes of myocardial tissue were observed by hematoxylin-eosin (HE) staining. The myocardial tissue was detected by immunohistochemistry (IHC) and westernblotting (WB). Interleukin-1(IL-1), transformation growth factor-β1(TGF-β1), nuclear factor-Kappa B(NF-κB) protein expression. Results The immunohistochemical results of myocardial tissue in rats showed that the absorbance values of IL-1, TGF-β1 and NF-κB protein were higher than those of the normal control group (P<0.05 or P<0.01). Compared with the simple irradiation group, IL-1, TGF-β1, NF-κB protein absorbance decreased (P<0.05). The expression of IL-1, TGF-β1 and NF-κB protein was increased in the myocardial tissue of rats compared with the normal control group (P<0.05). Compared with the simple irradiation group, IL-1 and TGF-β1 were compared. NF-κB protein expression decreased (P<0.05). Conclusson OPC can reduce the expression of IL-1, TGF-β1, NF-κB protein in myocardial tissue of RIHD rats, and prevent RIHD caused by radiotherapy, and the curative effect is better than blueberry.

Key words: Oligomeric proantho cyanidins (OPC), Blueberries, Radiation-induced heart disease (RIHD), Rat, Interleukin-1(IL-1), Transformation growth factor-β1(TGF-β1), Nuclear factor-Kappa B (NF-κB)

CLC Number:

Q95-33

XU Xing-jun, WU Fei, SONG Jin-yi, GAO Shi-le. Effects of Proanthocyanin on Expression of IL-1, TGF-β1 and NF-Kappa B Proteins in Rats with Radiation-induced Heart Disease[J]. Laboratory Animal and Comparative Medicine, 2019, 39(5): 383-389.

References

[1] 徐利明, 陈曦, 苑亚静, 等. 放射性心脏损伤的研究进展[J]. 中华放射肿瘤学杂志, 2017, 26(3):358-363.
[2] 刘惠惠, 李险峰. 放射性心脏损伤诊断和防治的临床研究进展[J].国际放射医学核医学杂志, 2016, 40(5):374-378.
[3] Chen J, Mehta JL.AngiotensinⅡ-mediated oxidative stress and procollagen-1 expression in cardiac fibroblasts:blockade by pravastatin and pioglitazone[J]. Am J Physiol Heart Circ Physiol, 2006, 291(4):H1738-1745.
[4] 沈伟生, 夏德宏, 高春恒, 等. 参松养心胶囊加红景天干预放射性心脏损伤的临床研究[J]. 南京中医药大学学报, 2016, 32(5):431-433.
[5] 姚晔, 钟亚华, 谢丛华. 放射性心脏损伤心肌纤维化发病机制研究进展[J]. 中华放射肿瘤学杂志, 201, 26(5):598-602.
[6] 储水鑫. 肺癌放疗并发症的中药治疗[J]. 中国中西医结合外科杂志, 2011, 17(6):645-646.
[7] 罗晓玲, 徐嘉红, 杨武斌, 等. 蓝莓花色苷抗氧化功能及稳定性研究进展[J]. 食品工业科技, 2018, 39(4):312-317.
[8] 张玉彪, 柳云恩, 佟昌慈, 等. 蓝莓花色苷对放射性心脏损伤的保护机制研究[J].临床急诊杂志, 2014, 15(11):647-650.
[9] 李聪, 周玉涛, 朱雪松, 等. 原花青素的提取分离及药理作用研究进展[J]. 国际中医中药杂志, 2017, 39(3):285-288.
[10] 马金柯, 高世乐, 胡宗涛, 等. 大鼠放射性心脏损伤机制的比较医学意义[J].实验动物与比较医学意义, 2018, 38(3):188-193.
[11] 刘丽娜, 武亚晶, 高光斌, 等. PDTC通过抑制NF-κB及下游通路减轻大鼠急性RIHD[J]. 中华放射肿瘤学杂志, 2018, 27(1):101-106.
[12] 马欣, 王丽, 芶芳芳, 等. 伊马替尼对尿毒症心肌病大鼠纤维化的保护作用[J]. 重庆医学, 2016, 45(3):313-316.
[13] 高世乐, 胡宗涛, 秦峰, 等. Smads蛋白家族在大鼠放射性肝纤维化形成过程中的表达[J]. 实验动物与比较医学, 2015, 35(3):188-194.
[14] 李晓燕, 韩霞, 张红明, 等. 耐力运动训练通过SIRT1信号通路保护力竭运动大鼠心肌[J]. 中华心血管病杂志, 2017, 45(6):501-506.
[15] Schwartz RS.Ionizing radiation and the coronary arteries:the plot thickening![J]. Catheter Cardiovasc Interv, 2007, 70(3):366-367.
[16] 刘阳, 高世乐, 胡宗涛, 等. 不同剂量单次照射下大鼠放射性心脏损伤模型的构建与评价[J]. 安徽医科大学学报, 2018, 53(5):716-721.
[17] 武亚晶, 王军, 王媛, 等. 慢性间歇性低压低氧预处理可减轻大鼠放射性心脏损伤[J]. 中华放射肿瘤学杂志, 2017, 26(2):222-227.
[18] 王祎, 王军, 程云杰, 等. 血清细胞因子TGF-β1和IL-1β表达水平对急性放射性心脏损伤发生的影响[J]. 中华放射医学与防护杂志, 2012, 32(5):488-491.
[19] 刘丽娜, 王升, 武亚晶, 等. NF-κB及下游通路与大鼠急性期放射性心肌纤维化相关性研究[J]. 2017, 26(4):453-458.