Enzyme-Linked Immunosorbent Assay for Detection of Virus Antibody in Golden Hamster

Abstract

Abstract: An enzyme-linked immunosorbent assay （ELISA） was developed for the detection of antibodies to Lymphocytic Choriomeningitis virus,Sendai virus，Pneumonia virus of Mouse and Reovirus type Ⅲ by using Horseradish Peroxidase （HRP） conjugates of rabbit antibody against hamster IgG （HRP-IgG） in golden hamster sera. The results were compared with HRP conjugates of Staphylococcus proteinA（HRP - SPA） ELISA. The specificity and sensitivity of the HRP-IgG ELISA are higher than those of the HRP-SPA ELISA. Therefore,it is recommended that the HRP-IgG ELISA procedure could be used as a routine method for the monitoring of viral infections in hamster colonies.

Key words: Golden Hamster, Enzyme-Linked Immunosorbent Assays, Virus, Antibody

YING Xian-Ping,QIAN Qin,QU Ya-Qin,et al. Enzyme-Linked Immunosorbent Assay for Detection of Virus Antibody in Golden Hamster[J]. Laboratory Animal and Comparative Medicine, 2000, 20(1): 34-36.

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Enzyme-Linked Immunosorbent Assay for Detection of Virus Antibody in Golden Hamster

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