Abstract:

Objective To understand the testing level of the relevant laboratories and standardize the detection of porcine parvovirus (PPV) nucleic acid by implementing laboratory proficiency testing plan of the PPV nucleic acid testing. Methods From January to August 2022, NIFDC organized the laboratory proficiency testing plan for PPV nucleic acid detection (plan number: NIFDC-PT-364). According to relevant CNAS standards, inactivated PPV was added into the serum of PPV negative pigs to prepare proficiency testing samples. After passing the homogeneity and stability tests, the samples should be distributed to the participating laboratories. Each laboratory was distributed 3 samples and was required to conduct qualitative detection of PPV nucleic acid, and submit the results and relevant original records within the specified time. Finally, the results submitted by each laboratory were summarized and analyzed. Results A total of 9 laboratories registered to participate in this proficiency testing, and all of them fed back the results within the specified time. Among them, the results of 8 laboratories met the expectations, accounting for 88.9%. Three samples from one laboratory were all tested positive for PPV, which was not consistent with the expected results, and it was judged as unsatisfactory. Most laboratories adopted real-time fluorescent PCR detection method, and used domestic reagents for nucleic acid extraction, while the PCR amplification reagents were mainly imported reagents. The use of different brands of reagents didn't have impact on the detection results. There were some problems in some laboratories, such as unclear diagram of experimental results and incomplete information of original records. Conclusion Most of the participating laboratories are capable of PPV nucleic acid detection, but the detection details need to be improved.

Key words: Porcine parvovirus, Proficiency testing, Nucleic acid, PCR