›› 2000, Vol. 20 ›› Issue (1): 34-36.

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Enzyme-Linked Immunosorbent Assay for Detection of Virus Antibody in Golden Hamster

  

  1. Shanghai Institute of Biological Products,Shanghai 200051
  • Received:1999-04-18 Online:2000-01-25 Published:2013-03-19

Abstract: An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies to Lymphocytic Choriomeningitis virus,Sendai virus,Pneumonia virus of Mouse and Reovirus type Ⅲ by using Horseradish Peroxidase (HRP) conjugates of rabbit antibody against hamster IgG (HRP-IgG) in golden hamster sera. The results were compared with HRP conjugates of Staphylococcus proteinA(HRP - SPA) ELISA. The specificity and sensitivity of the HRP-IgG ELISA are higher than those of the HRP-SPA ELISA. Therefore,it is recommended that the HRP-IgG ELISA procedure could be used as a routine method for the monitoring of viral infections in hamster colonies.

Key words: Golden Hamster, Enzyme-Linked Immunosorbent Assays, Virus, Antibody