Abstract: Objective    To detect the distribution of adenovirus type 5 (Ad5) in C57BL / 6J infected by four different routes. Methods    One hundred and twelve C57BL / 6J mice were divided into 4 groups, each mouse was given 100 µL of Ad5 solution (1 × 109 PFU / mL) by intranasal drip, intragastric administration, tail vein injection and intraperitoneal injection.  On the 1 st, 2 nd, 3 rd, 4 th, 5 th, 6 th and 7 th day after infection, two males and two females in each group were sacrificed for the real-time PCR quantitative detection of Ad5 content in lymph nodes, testis, epididymis, uterus, ovary, spleen, kidney, liver, lung, heart, brain, blood and bone marrow. Results    Ad5 was detected in lung and spinal cord tissue in intranasal drip group, the content of Ad5 in lung decreased mostly 4 days after infection; Ad5 was not detected in all tissues in intragastric administration group; Ad5 was detected in liver and spleen in tail vein injection group, and the decline rate was slow; Ad5 was detected in spinal cord, spleen, epididymis and testis in intraperitoneal injection group, the content of Ad5 in the spinal cord was about 10 times higher than that in the intranasal drip group, the content of Ad5 in spleen was similar to that in tail vein injection group, and the content of Ad5 in epididymis and testis was only 1% ~ 10% of that in other positive tissues. Conclusion    The lung infection test of Ad5 can be carried out by intranasal drip once every 3 days, the liver infection test by tail vein injection, the spleen infection test by tail vein injection or intraperitoneal injection, and the spinal cord infection test by intraperitoneal injection.

Key words: Adenovirus type 5, Real-time PCR, Intranasal drip, Intragastric administration, Tail vein , injection, Intraperitoneal injection, Mice