Abstract: Objective To establish an effective PCR assay for detection of cilia-associated respiratory bacillus, and applicate the method for detection in mouse. Methods Sequence of CAR bacillus was obtained from NCBI genbank. A plasmid was structured by selecting the appropriate sequence. And primers were designed based on the sequence; the positive amplified fragments were sequenced to verify the reliability of the method. Results The PCR method for detection of CAR bacillus was successfully established. Conclusion The establishment of the method will help the detection of experimental animals for CAR bacillus.

Key words: Cilia-Associated Respiratory Bacillus, PCR, Application