Genetic Analysis of Rhesus Macaques(Macaca mulatta) Using Microsatellite Markers

doi:10.3969/j.issn.1674-5817.2014.02.010

Abstract

Abstract: Objective Inbreeding is the big problem in captive colonies of rhesus macaques.To preserve genetic variability, the exact pedigree must established by genetic markers. Methods Fourteen autosomal short tandem repeat (STR) loci with high information content were used to 64 rhesus macaques by fluorescently-labeled primer polymerae chain reaction (PCR) and capillary electrophoresis typing. Then, these loci were evaluated by unique genetic characterization, parentage determination, and estimation of parameters. Results Seven of the 14 markers have high gene diversity (H_e) and polymorphic information content (PIC)(>0.8), the combined probability of genetic identity (CPI) at all loci is 1.21E-18, and the combined probability of no-parent exclusion (CPE1) or single parent exclusion (CPE2) at all loci can reach 0.9985 and 0.9999 respectively. Conclusion These markers are reliable and highly informative genetic markers, can provide for powerful parentage determination, unique genetic characterization, and accurate estimation of parameters, which are all useful for genetic management of rhesus macaques.

Key words: Rhesus macaques, STR, Genetic management

CLC Number:

Q95-33

YANG Na, LIU Xiao-shuai, ZHOU Liang, ZENG Dai-wen. Genetic Analysis of Rhesus Macaques(Macaca mulatta) Using Microsatellite Markers[J]. Laboratory Animal and Comparative Medicine, 2014, 34(2): 131-135.

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