Application of Caesarean and in vitro Fertilization for Bio-cleansing in Mice

doi:10.3969/j.issn.1674-5817.2013.03.011

Abstract

Abstract: Objective Caesarean and in vitro fertilization were used to rederive the contaminated SPF mouse colonies. Methods The contaminated mice with normal reproductive capacity were hysterectomized according to the vaginal plugs and abdomen palpatiion after natural mating. The fetus was removed from the uterus when labor and the pups were suckled by foster mothers. The foster nursing and the survival rates of the pups were observed. The in vitro fertilization and embryos transfer was used to the mouse strains whose reproductive capacity was poor or breeding failure. Both sperm and the eggs were incubated in the HTF medium, and 3.5 days embryos were transferred to the uterus of SPF recipient mice. Results All the re-derived mice through both methods reached the standard of SPF animals. Conclusions For the mice which did not meet the SPF standard but pathogens on them could not vertically transmit; the two methods can effectively improve their microbial level.

Key words: Caesarean, In vitro fertilization, Embryos transfer, Bio-cleanse

CLC Number:

Q95-33

ZHU Lian, KONG Peng-Cheng, WANG Mei-Shan, CHEN Xue-Jin, JIANG Man-Xi, CUI Li. Application of Caesarean and in vitro Fertilization for Bio-cleansing in Mice[J]. Laboratory Animal and Comparative Medicine, 2013, 33(3): 218-221.

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