Abstract: Objective To Clone p-defensin gene in Mongolian gerbil. Methods The primers was designed according to the published sequences in GenBank. A RT-PCR method was applied to clone the defens in beta 1 gene in small intestine of the Mongolian gerbil. The PCR products was sequenced and blasted with related sequences. Results The sequenced results presented that the product was highly identical to the defens in beta 1 gene in rat and mouse. The identification between the present sequence and that of rat or mouse was ranged from 85% to 78%，the GenBank Accession Number is EU784838. Conclusions The acquired sequence was a portion of defens in beta 1 gene, which presented highly identical to the same gene in rat and mouse.

Key words: Mongolian gerbil, β-defensin, Cloning, Sequence analysis, Identification