Application of PCR Method for Rapid Sex Determination in SPF Jinding Duck

doi:10.3969/j.issn.1674-5817.2017.03.016

Abstract

Abstract: Objective To determine the sex of SPF Jinding Duck, the universal primer gCHD of avian chromosomal DNA-binding protein 1 (CHD1) gene on avian chromosomes were synthesized. Methods Blood of 85 SPF Jinding Duck were collected and genomic DNA was extracted for PCR amplification. The sex of the birds were determined by analyzing the number of bands after gel electrophoresis. Results The CHD1-Z and CHD1-W were found in the PCR products of agarose gel electrophoresis from females and CHD1-Z was from males. Conclusions The PCR can be used as an effective method for sex identification in birds.

Key words: SPF Jinding ducks, Sex identification, PCR

CLC Number:

Q95-33

XU Li-jing, SUN Chang, LU Tao-feng, CHEN Hong-yan, ZHAO Li-li. Application of PCR Method for Rapid Sex Determination in SPF Jinding Duck[J]. Laboratory Animal and Comparative Medicine, 2017, 37(3): 249-251.

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