Development of a Loop-mediated Isothermal Amplification Assay for Detection of Corynebacterium bovis

doi:10.3969/j.issn.1674-5817.2019.05.003

Abstract

Abstract: Objective To establish a rapid and specific loop-mediated isothermal amplification (LAMP) assay for Corynebacterium bovis(C.bovis). Methods A set of specific primers were designed based on the 16S rRNA of C. Bovis. The reaction parameters of LAMP assay were optimized and the specificity and sensitivity were determined. Results Among the tested bacteria, only C.bovis could be detected by the LAMP assay, no other bacteria were positive. The detection limit of the LAMP assay was 118 fg/μL genomic DNA of C. bovis. Conclusions The detection could be completed without expensive equipment and it was simple, specific, sensitive, and the result was ready to read-out. LAMP could be a good alternative method for a rapid detection and surveillance of C. bovis.

Key words: Corynebacterium bovis (C.bovis), Loop-mediated isothermal amplification (LAMP), Detection

CLC Number:

Q95-33

FENG Jie, ZHANG Quan, QIAN Miao, XIE Jian-yun, GAO Cheng. Development of a Loop-mediated Isothermal Amplification Assay for Detection of Corynebacterium bovis[J]. Laboratory Animal and Comparative Medicine, 2019, 39(5): 349-355.

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