Development of Detection Method for Sendai Virus by Nucleic Acid Sequencing

doi:10.3969/j.issn.1674-5817.2017.03.006

Abstract

Abstract: Objective To develop the method for detection of Sendai virus by nucleic acid sequencing. Methods General primers according to Sendai virus genome sequences were designed and optimized to sequencing primers. Then the best condition of creating library was established. This method was verified by nucleic acid sequencing and analysis. Results A pair of highly specific general primers was designed. The sequences are respectively 5'-GCTGCAAAACGCTGTGGG-3' and 5'-TGGRACYT-CAGAAAGAATRGG-3', and the sequencing primers were established by ligated adaptor sequences to the 5'-end of general primers. The established condition of creating library and library products were obtained by the first round amplification using general primers and the second round using sequencing primers. Sendai virus was effectively distinguish from other organisms by nucleic acid sequencing and analysis, and was precised to TianJin strain. Conclusion A detection method was developed for Sendai virus by nucleic acid sequencing, which may be as the base for microbiological detection in laboratory animals.

Key words: Sendai virus, Nucleic acid sequencing, Detection

CLC Number:

Q95-33

ZHANG Huan-huan, YU Chen-huan, DAI Fang-wei, MA Yue, LANG Ju-lia, WANG Yu, YING Hua-zhong. Development of Detection Method for Sendai Virus by Nucleic Acid Sequencing[J]. Laboratory Animal and Comparative Medicine, 2017, 37(3): 204-208.

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