Abstract: Objective To investigate the infection status of intestinal Amoeba in diarrhea tree shrews from the center of germplasm resources, to find out the cause of diarrhea, and to provide useful reference and support for prevention and treatment of parasitic infection in tree shrews. Methods Totally 78 samples were collected from the fecal and blood of diarrhea tree shrews, and then were detected by the normal saline direct smear method and iodine solution staining, meanwhile stool antigen and IgG antibody in serum were detected by ELISA of Amoeba. Results The results showed that the positive infection rate were 42.22%(19/45) of wild tree shrews, 33.33%(11/33) of breeding of tree shrews by direct smear and iodine staining method. The infectious rates of E. histolytica were 64.44%(29/45) and 42.42%(14/33)respectively when detected the stool antigen by ELISA. The positive rate of Amoeba IgG were 66.67%(30/45) and 63.63%(21/33) by ELISA detection. Conclusions Amoeba infection rate is rather high in tree shrews, and it may be one of the pathogens of diarrhea tree shrews. In routine breeding should be strengthened in the process of testing control the spread of the parasite.

Key words: Tree shrews, Amoeba, Morphological detection, ELISA detection