The Expression of Myelin Related Inhibitory Factor in Microenvironment after Spinal Cord Injury in SD Rats

doi:10.3969/j.issn.1674-5817.2018.05.003

Abstract

Abstract: Objective To observe the expression of myelin related inhibitory factor in Sprague-Dawley (SD) rats at different time points in different injury potential energy of Allen's model and spinal cord total cut model with the microenvironment. Methods One hundred and twenty five adult female healthy SD rats were selected and randomly divided into 5 groups with 25 in each, group A (sham operation group), group B [(20 g×5 cm, injury potential energy 100 gram-cm force (gfc)], group C (20 g×10 cm, injury potential energy 200 gcf) , group D (20 g×15 cm, injury potential energy 300 gcf); group E (total spinal cord resection group).The expression of myelin related inhibitory factors of neurite outgrowth inhibitor-A (Nogo-A), the myelin associated glycoprotein(MAG), and the oligodendrocyte myelin glycoprotein (OMgp) were analyzed by Western blotting after spinal cord injury (SCI) at 1 d, 5 d, 7 d, 14 d and 28 d after successful modeling. Results Twenty-four cases (16.67%) of SD rats were died in the whole experiment. The expression of Nogo-A, MAG and OMgp was observed at the first day after SCI in group B, C, D and E. The expression of Nogo-A and MAG reached the peak at the 7th day and returned to the levels of sham operation group on the 28th day. The expression of OMgp in myelin was observed at the first day after SCI, and reached the peak at day 5, and returned to the levels of sham operation group on the 28th day. Conclusion The Nogo-A, MAG and OMgp are highly expressed after SCI and those indexes are reached the peak 5-7 days after SCI.

Key words: Spinal cord injury (SCI), SD rat, Allen's model, Myelin related inhibitory factor

CLC Number:

Q95-33

JIA Xu-feng, Long Miao, JI Yong, HUANG Guang-ping, ZHOU Yu, FENG Da-xiong. The Expression of Myelin Related Inhibitory Factor in Microenvironment after Spinal Cord Injury in SD Rats[J]. Laboratory Animal and Comparative Medicine, 2018, 38(5): 336-342.

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