Abstract: Objective To monitor the bacteria in the pipe network and the water used in microbiological sterile water system in barrier breeding facilities, and to observe the effect of drinking micro-electrolysis sterile water on blood biochemical in SPF-grade SD rats. Methods From the period of 2015 to 2017, 6 outlet points of the micro-electrolysis water system were sampled and detected; 21-day-old SD 20 rats were divided into 2 groups, half male and half female, drinking micro-electrolyzed water and high pressure water respectively. After drinking Sterilized water 1 to 8 months, Blood was taken from the abdominal aorta to detect blood biochemical. Results The microelectrolytic water system has been detected for more than 2 years. Using the current detection technology, no bacteria have been detected in the micro-electrolyzed water and the pipe network. There was no difference in blood biochemical indicators after drinking 1 to 8 months of water in 2 groups. Conclusion The application of micro-electrolysis sterile water system in barrier breeding facilities has a continuous disinfection and bactericidal effect on the pipe network and the water, and can kept sterile for a long time. No effect on blood biochemical indicators were observed in 21-day-old SD rats drinking micro-electrolyzed water for 8 months .

Key words: Microelectrolysis water system, Sterile, SD rats, Biochemical indicator