Comparative Study on Formation of Atherosclerosis and Phospholipase A2 Expression in ZDF and SD Rats

doi:10.3969/j.issn.1674-5817.2015.01.003

Abstract

Abstract: Objective To observe the formation of Atherosclerosis(AS) and expression of cPLA2, sPLA2, LP-PLA2 in AS tissue of ZDF rats and SD rats. Methods 6 ZDF (fa/fa) rats and 6 SD rats, with high-fat feeding and small dose, multiple times, intraperitoneal injection of 7×10⁵ U/kg vitamin D3,to build ZDF rats AS model group and SD rats AS model group, 6 ZDF (fa/+) rats and 6 SD rats, respectively,as ZDF rats control group and SD rats control group, fed with normal diet. GLU, TC, TG, HDL-C, LDL-C were measured before and after the establishment of the model, blood Ca²⁺ was measured after the establishment of the model, HE staining and Real-time PCR to detect the expression of cPLA2, sPLA2, LP-PLA2 mRNA in the abdominal aorta. Results After the establishment of the model, fasting blood glucose, blood lipid and blood Ca²⁺ were significantly higher (P<0.01), typical AS and obvious calcification were appeared in abdominal aorta of ZDF rats AS model group and SD rats AS model group, fasting blood glucose, blood lipid of ZDF rats AS model group were significantly higher than SD rats AS model group, and AS lesion was relatively serious, in ZDF rats AS model group, the expression of cPLA2, sPLA2 were significantly increased (P<0.01), while the expression of LP-PLA2 was significantly decreased (P<0.01); in SD rats AS model group, the expression of cPLA2, sPLA2, LP-PLA2 were significantly increased (P<0.01). Conclusions With high-fat feeding and small dose, multiple times, injection of vitamin D3 can establish the AS model in ZDF rats and SD rats, and AS lesion is relatively serious in ZDF rats, the expression of PLA2s are different in ZDF rats and SD rats, and this may be related to molecular pathological mechanism of the formation of AS in ZDF rats and SD rats.

Key words: ZDF RAT, SD rat, High fat feeding, Vitamin D3, Atherosclerosis (AS), PLA2

CLC Number:

Q95-33

SI Xu-wei, XI Sai-fei, XU Xiao-ping, ZHUGE Fu-yuan, CHEN Xiao-zhen, CAI Yue-qin, CHEN Min-li. Comparative Study on Formation of Atherosclerosis and Phospholipase A2 Expression in ZDF and SD Rats[J]. Laboratory Animal and Comparative Medicine, 2015, 35(1): 10-16.

References

[1] Six DA, Dennis EA.The expanding superfamily of phospholipase A2 enzymes: classification and characterization[J]. Biochimi Biophys Acta, 2000, 1488(1-2):1-19.
[2] 赵娟, 李相军, 孙波, 等. 维生素D3 联合高脂饲料建立大鼠动脉粥样硬化模型[J]. 实用医学杂志, 2009, 25(21): 3569-3571.
[3] 温进坤, 韩梅, 杜玮南, 等. 一种快速建立大鼠动脉粥样硬化模型的实验方法[J]. 中国老年学杂志, 2001, 21(1): 50-52.
[4] 郭啸华, 刘志红, 李恒, 等. 高糖高脂饮食诱导的 2 型糖尿病大鼠模型及其肾病特点[J]. 中国糖尿病杂志, 2002, 10(5): 290-294.
[5] Schmid K, McSharry WO, Pameijer CH, et al. Chemical and physicochemical studies on the mineral deposits of the human atherosclerotic aorta[J]. Atherosclerosis, 1980, 37(2): 199-210.
[6] Fleckenstein-Grün G, Thimm F, Luley C, et al.Differentiation between calcium-and cholesterol-dominated types of arteriosclerotic lesions: antiarteriosclerotic aspects of calcium antagonists[J]. J Cardiovasc Pharmacol, 1991, 18:1-9.
[7] 吴开云, 高摄渊. 维生素D诱发大鼠动脉粥样硬化的实验研究[J]. 解剖学报, 1996, 27(2):133-135.
[8] Bennani-Kabchi N, Kehel L, El Bouayadi F, et al.New model of atherosclerosis in insulin resistant sand rats: hypercholesterolemia combined with D2 vitamin[J]. Atherosclerosis, 2000, 150(1):55-61.
[9] Ross R.Atherosclerosis-an inflammatory disease[J]. N Engl J Med, 1999, 340(2):115-126.
[10] Devaraj S, Xu DY, Jialal I.C-reactive protein increases plasminogen activator inhibitor-1 expression and activity in human aortic endothelial cells implications for the metabolic syndrome and atherothrombosis[J]. Circulation, 2003, 107(3):398-404.
[11] Ghesquiere S, Gijbels M, Anthonsen M, et al.Macrophage specific overexpression of group IIa sPLA2 increases atheroscIerosis and enhances collagen deposition[J]. J Lipid Res, 2005, 46(2):201-210.
[12] 王晓辉, 颜光涛. cPLA2α炎性反应中信号转导的“重量级”分子[J]. 中国危重病急救医学, 2004, 16(6):378-380.
[13] 郭朝阳,隋少玉,郭朝晖,等. 脂蛋白脂酶活性与老年 2 型糖尿病关系的研究[J]. 中国老年学杂志,2004,24(6): 510-511.
[14] 杨振华, 王瑞英, 仝志强, 等. 2 型糖尿病患者餐后血脂反应及脂蛋白脂酶活性变化的研究[J]. 临床荟萃, 2008, 23(3):169-173.
[15] Tan G D, Olivecrona G, Vidal H, et al.Insulin sensitisation affects lipoprotein lipase transport in type 2 diabetes: role of adipose tissue and skeletal muscle in response to rosiglitazone[J]. Diabetologia, 2006, 49(10):2412-2418.