Serological and Viral Replication of Murine Norovirus in ICR Mice with Different Routes of Artificial Infection

doi:10.3969/j.issn.1674-5817.2018.04.006

Abstract

Abstract: Objective To investigate the serum antibody levels and viral replication of murine norovirus (MNV)in mice by artificial infecting in a variety of ways.Method ICR mice were inoculated with MNV (4 ×10⁴ copies/µL) respectively through intravenous combining with intraperitoneally (iv+ip group),drinking water (dw group),intragastrical injection (ig group).Heart,liver,spleen,lung,kidney,brain,gastrointestinal contents samples and serum were taken on 0,3,6,14,25,34,51 day postinfection (dpi) (2~3 mice each time).qRT-PCR and ELISA method were applied to detect virus RNA and serum antibody.Results Asymptomatic infection has been identified in all mice.The specific antibody can be first detected 25 dpi in three groups.The level of antibodies was the highest in the ig group in early times,then antibody levels of the iv+ip group rose faster 51 dpi than the other groups.MNV RNA can be detected 3 dpi in the gastrointestinal tract,spleen and brain.Different viral load in each organization among groups.The viral load of cecum contents (3~ 51 d),spleen (6 d) and liver (14 d) viral load in the iv+ip group is higher than the other two groups.Conclusion The effects of different inoculation methods on the antibody level and virus replication of MNV were significant.The effect of infection combined intravenous with intraperitoneally come out to be good,which can be used as MNV effective inoculation for animal model.Serum antibody ELISA detection combined with cecum contents nucleic acid detection is advantageous to the MNV early monitoring.

Key words: Murine norovirus (MNV), Serological, Viral replication, Infection, Approaches

CLC Number:

Q95-33

LUO Yin-zhu, ZHANG Yu, YUAN Wen, HE Li-fang, HUANG Bi-hong, WU Rui-ke, Min Fan-gui, WANG Jing, PAN Jin-chun, HUANG Ren. Serological and Viral Replication of Murine Norovirus in ICR Mice with Different Routes of Artificial Infection[J]. Laboratory Animal and Comparative Medicine, 2018, 38(4): 272-277.

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