Abstract:  Objective    To establish an in vitro test method for acute eye irritation of disinfection products by using porcine cornea. Methods    In vitro detection conditions on porcine corneal system were investigated and optimizated, which used ethanol and imidazole as positive control. Results    It was confirmed by liquid and solid disinfectants that after the exposure of ethanol for 10 min and incubation for 80 min, or the imidazole exposure for 3 h and incubation for 70 min, the in vitro stimulation score was within the standard range of bovine corneal opacity and permeability (BCOP) corresponding positives, and the test system was established. Conclusion    A method for detecting acute eye irritation of disinfectants is initially established by using pig eyes instead of bovine eyes, which can provide a reference for the safety evaluation of disinfection products.

Key words: Disinfection products, Porcine cornea, Bovine cornea, Eye irritation