Establishment and Preliminary Application of RT-PCR Method for Detection of Bovine Parainfluenza Virus Type3 in Bovine Origin Samples

doi:10.3969/j.issn.1674-5817.2018.02.004

Abstract

Abstract: Objective To establish a RT-PCR method for detection of bovine parainfluenza virus type3 (BPIV3) in bovine origin samples.Methods The primers were designed and synthesized according to the published BPIV3 specific sequences of neuraminidase (HN) gene.RT-PCR method was established,and the specificity,sensitivity,repeatability and stability of this method were tested.Then the method was used to detect 137 bovine origin samples.Results The developed RT-PCR method was no cross reaction with bovine viral diarrhea virus (BVDV),bovine herpesvirus type1(BHV-1),Sendai virus(SV).The lowest detection virus titer is 10^-6/mL.BPIV3 cDNA maintained at -30 ℃ for 12 months,still can enlarge the fragment of about 610 bp visible purpose belt .The nucleic acid positive rate of 137 bovine origin samples were 14.6% about BPIV3 detected by RT-PCR.Conclusion The developed RT-PCR method is good in specificity,sensitivity,stability,and can be used for detecting the BPIV3 in bovine origin samples.

Key words: Bovine parainfluenza virus type3 (BPIV3), PCR, Bovine origin sample

CLC Number:

R372Q95-33

WANG Ji, FU Rui, LI Xiao-bo, WANG Shu-jing, WANG Sha-sha, LI Wei, QIN Xiao, GONG Wei, YUE Bing-fei, HE Zheng-ming. Establishment and Preliminary Application of RT-PCR Method for Detection of Bovine Parainfluenza Virus Type3 in Bovine Origin Samples[J]. Laboratory Animal and Comparative Medicine, 2018, 38(2): 98-104.

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