Preliminary Probing for Reproductive Performance in Female Dwarfism Rats

doi:10.12300/j.issn.1674-5817.2020.166

Abstract

Abstract: Objective To probe the causes of low reproductive performance in spontaneous dwarfism Wistar rats (SDWR). Methods A Wistar rat control group and a SDWR group both included 50 female and 50 male rats aged 12 weeks respectively, and were mated 1∶1 (male to female); the pregnancy rate, litter size, litter birth weight, weaning rate and weaning weight of the SDWR and Wistar rats were measured from the first parity to the fourth. Twenty female SDWR aged 8 weeks and 20 female Wistar rats aged 5 weeks were selected respectively to evaluate puberty and estrous cycle by vaginal cytology. Twenty female SDWR and 20 female Wistar rats aged 6-8 weeks were respectively injected intraperitoneally with 200 U/kg, 300 U/kg and 400 U/kg of pregnant mare serum gonadotropin (PMSG) plus human chorionic gonadotropin (hCG) for superovulation, and the ovulation induction effects. Twenty Wistar, 20 SDWR and 20 SDWR superovulation rats aged 6-8 weeks were compared in morphological changes of ovarian tissue by HE staining. Results The pregnancy rate, litter size, litter birth weight, weaning weight and weaning rate of the first parity to the fourth of the SDWR group were significantly lower than those of the Wistar group (P<0.05). In the SDWR group, sexual maturation [(12.15 ± 0.81) weeks] was significantly delayed compared with the Wistar group [(8.05±0.76) weeks]. The Wistar group maintained a regular estrous cycle of 4-5 days, while the SDWR group showed an extended and disordered estrous cycle of 7-8 days, shortened estrous and extended metestrus. The results of superovulation test showed that the number of eggs in the SDWR group was significantly lower than that in the Wistar group (P<0.01). The results of HE staining of ovarian tissue showed that in the Wistar group, follicles at all levels were growing actively, and granular cells and corpus luteum structure were normal; while in the SDWR group, the number of total ovarian follicles and follicles at all levels were significantly reduced, and granular cells was disarranged. Conclusion The low reproductive rate of SDWR may be related to delayed sexual maturity, prolonged and disordered estrous cycle, and ovarian dysfunction.

Key words: Dwarfism rats, Reproductive performance, Estrous cycle, Ovarian dysfunction

CLC Number:

Q95-33

JIAO Shufan, XU Longmei, HUA Zhengyu, YAO Jufang. Preliminary Probing for Reproductive Performance in Female Dwarfism Rats[J]. Laboratory Animal and Comparative Medicine, 2021, 41(1): 40-45.

Figures/Tables 2

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