Construction and Evaluation of Chromosome Substitution Strains

doi:10.3969/j.issn.1674-5817.2015.06.010

Abstract

Abstract: Objectives To shorten the fine-structure mapping time, construct and evaluate the programme of chromosome substitution strains (CSSs) . Methods The rst step required making hybrids between C57BL/6 male mice and donor female mice. N1 hybrids male mice were backcrossed to host C57BL/6 female mice. Progeny with a non-recombinant chromosome derived from donor strain, in this case chromosome 1, were identified in this and subsequent backcrosses. N2 Male mice were backcrossed to host C57BL/6 female mice at each generation. A certain number of progeny per generation was screened with short tandem repeat (STR) and single nucleotide polymorphism (SNP). These mice were backcrossed to host C57BL/6 at each generation until tenth generation. At the tenth backcross generation males and females with the non-recombinant chromosome derived from donor mice were intercrossed. Progeny of this intercross that were homozygous (homosomic) for chromosome 1 were used to propagate the homosomic strain. The homozygosity of CSSs was identified through scanning the whole genomic by microarray to compare with expected proportion. Results We have constructed two CSSs by the programme that had been constructed. The actual homozygosity of progeny with microarray was approaching the expected. Conclusion The constructed CSSs confirmed the replacement of target chromosome and over 99.59% chromosome segments from host C57BL/6. This breeding program to generate a CSSs panel proved to be feasible.

Key words: Chromosome substitution strains (CSSs), Construct the programme, Discuss, Rate of non-recombination

CLC Number:

Q95-33

ZHAO Ying, ZHAO Li-ya, CHAO Tian-zhu, ZHANG Rong, XING Zheng-hong, CHEN Guo-qiang, XIAO Jun-hua. Construction and Evaluation of Chromosome Substitution Strains[J]. Laboratory Animal and Comparative Medicine, 2015, 35(6): 478-483.

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