Screening of Mouse Strain Susceptive to Chronic Stress Depression and Preliminary Research on Its Mechanism

doi:10.3969/j.issn.1674-5817.2016.04.003

Abstract

Abstract: Objective To screen mouse strain that susceptive to chronic unpredicted mild stress(CUMS) and preliminarily research its susceptive mechanism. Methods KM, ICR, BABL/c and C57BL/6 mice were used to establish CUMS depression model. Body weight, sucrose preference test(SPT), open field test (OFT) were used to evaluate the sensitivity of mice to CUMS. Hippocampal levels of glucocorticoid reccptor (GR), 5-hydroxytryptamine(5-HT), kynurenine(KYN) were measured and used for susceptive mechanism research. Results Body weight, SPT, horizontal movement score and vertical movement score of C57BL/6 mice were found to susceptive to CUMS. SPT, horizontal movement score and vertical movement score of BABL/c showed similar tendency. Hippocampal levels of GR and KYN of normal C57BL/6 mice were significantly lower or higher than that of normal KM, ICR and BABL/c mice. Conclusions The C57BL/6 mouse is most susceptive to CUMS among 4 strains tested, and hippocampal levels of GR and KYN may be the main mechanism responsible for its susceptibility.

Key words: Mouse, Chronic stress, Susceptive strain, Mechanism

CLC Number:

R749.4Q95-33

XU Long-jin, WANG Kou-zhou. Screening of Mouse Strain Susceptive to Chronic Stress Depression and Preliminary Research on Its Mechanism[J]. Laboratory Animal and Comparative Medicine, 2016, 36(4): 257-262.

References

[1] 苏宝兰, 孙福刚, 云维生, 等. 抑郁症的性别差异[J]. 临床精神医学杂志, 2008, 18(2):97-98.
[2] Phillips MR, Zhang J, Shi Q, et al.Prevalence, treatment, and associated disability of mental disorders in four provinces in China during 2001-05: an epidemiological survey[J]. Lancet, 2009, 373(9680):2041-2053.
[3] 陈湘, 窦建卫, 胡亚丽. 抑郁症动物模型的应用及研究进展[J]. 中国组织工程研究与临床康复, 2008, 12(28):5521-5524.
[4] 许晶, 李晓秋. 慢性应激抑郁模型的建立及其评价[J]. 中国行为医学科学, 2003, 12(1):14-17.
[5] Reichardt HM, Umland T, Bauer A, et al.Mice with an increased glucocorticoid receptor gene dosage show enhanced resistance to stress and endotoxic shock[J]. Mol Cell Biol, 2000, 20(23):9009-9017.
[6] 夏军, 叶慧, 周义成, 等. 慢性应激大鼠抑郁模型的建立及其有效性的探讨[J]. 华中科技大学学报: 医学版, 2005, 34(4):493-495.
[7] 王建醒, 周丽, 徐华锋, 等. 慢性应激大鼠抑郁模型的建立及评价[J]. 齐齐哈尔医学院学报, 2006, 27(6):644-646.
[8] Dong H, Gao Z, Rong H, et al.β-asarone reverses chronic unpredictable mild stress-induced depression-like behavior and promotes hippocampal neurogenesis in rats[J]. Molecules, 2014, 19(5):5634-5649.
[9] Jiang P, Dang RL, Li HD, et al.The impacts of swimming exercise on hippocampal expression of neurotrophic factors in rats exposed to chronic unpredictable mild stress[J]. Evid Based Complement Alternat Med, 2014(2014):729827.
[10] Wang M, Chen Q, Li M, et al.Alarin-induced antidepressant-like effects and their relationship with hypothalamus-pituitary-adrenal axis activity and brain derived neurotrophic factor levels in mice[J]. Peptides, 2014, 56(3):163-172.
[11] Xu J, Xu H, Liu Y, et al.Vanillin-induced amelioration of depression-like behaviors in rats by modulating monoamine neurotransmitters in the brain[J]. Psychiatry Res, 2015, 225(3):509-514.
[12] Oldehinkel AJ, Bouma EM.Sensitivity to the depressogenic effect of stress and HPA-axis reactivity in adolescence: a review of gender differences[J]. Neurosci Biobehav Rev, 2011, 35(8):1757-1770.
[13] 颜冬梅, 屠凌岚, 李文钧. 糖皮质激素受体:抑郁症治疗的新靶点[J]. 中国现代应用药学, 2006, 23(7):620-622.
[14] 楼剑书, 李昌煜. 抑郁症受体机制研究进展[J]. 中国药理学通报, 2006, 22(10):1157-1160.
[15] 张国双, 杨坤. 抗抑郁新靶点-糖皮质激素受体[J]. 国际精神病学杂志, 2015, 42(1):84-86.
[16] 李金兵, 李翼鹏, 田俊生, 等. 基于慢性温和不可预知应激模型内源性代谢物变化探讨抑郁症病理机制[J]. 中草药, 2013, 44(1):108-115.
[17] Agudelo L, Femenía T, Orhan F, et al.Skeletal muscle PGC-1〈1 modulates kynurenine metabolism and mediates resilience to stress-induced depression[J]. Cell, 2014, 159(1):33-45.
[18] 洪煜, 张蕴琨. 脑组织中5-HT及受体与运动的研究现状[J]. 南京体育学院学报:自然科学版, 2008, 7(3):102-106.