A Modified Delivery of Mouse Cauda Epididymides at Room Temperature

doi:10.3969/j.issn.1674-5817.2014.02.013

Abstract

Abstract: Objective To explore the optimal conditions for Cauda epididymis transport under room temperature and eliminate the effect of medium on the transport of cauda epididymides at room temperature. Methods The transport methods of cauda epididymides were modified in this study and the sperm motilities, fertility and developmental abilities of IVF embryos after transportation at room temperatures or 4~8℃ in 20 μl of medium M2 or medium-free tube were compared. Results Under room temperatures, delivery of cauda epididymides without medium was significantly superior to with medium, but was inferior to 20 μl M2 medium under 4~8℃. Conclusion The modified medium-free system was beneficial to room temperature transportation of cauda epididymides and maybe eliminate the side effects of medium on spermatozoa, and the delivery of cauda epididymides under room temperature was useful for transporting some valuable strains between mouse banks and research facilities.

Key words: Cauda epididymis, Transport, Sperm viability, Motility, Embryo development

CLC Number:

Q95-33

WANG Mei-shan, KONG Peng-cheng, ZHU Yan, ZHU Lian, JIANG Man-xi, CHEN Xue-jin, LI He-ping. A Modified Delivery of Mouse Cauda Epididymides at Room Temperature[J]. Laboratory Animal and Comparative Medicine, 2014, 34(2): 145-148.

References

[1] Fuller SJ, Whittingham DG.Effect of cooling mouse spermatozoa to 4 degrees C on fertilization and embryonic development[J]. J Reprod Fertil, 1996, 108(1):139-145.
[2] Sato M, Ishikawa A.Room temperature storage of mouse epididymal spermatozoa: exploration of factors affecting sperm survival[J]. Theriogenology, 2004, 61(7-8):1455-1469.
[3] Sato M, Ishikawa A, Nagashima A, et al.Prolonged survival of mouse epididymal spermatozoa stored at room temperature[J]. Genesis, 2001, 31(4):147-155.
[4] Kaneko T, Fukumoto K, Haruguchi Y, et al.Fertilization of C57BL/6 mouse sperm collected from cauda epididymides after preservation or transportation at 4 degrees C using laser-microdissected oocytes[J]. Cryobiology, 2009, 59(1):59-62.
[5] Mochida K, Ohkawa M, Inoue K, et al.Birth of mice after in vitro fertilization using C57BL/6 sperm transported within epididymides at refrigerated temperatures[J]. Theriogenology, 2005, 64(1):135-143.
[6] Sankai T, Tsuchiya H, Ogonuki N.Short-term nonfrozen storage of mouse epididymal spermatozoa[J]. Theriogenology, 2001, 55(8):1759-1768.
[7] Jishage K, Ueda O, Suzuki H.Fertility of mouse spermatozoa from cauda epididymis preserved in paraffin oil at 4 C[J]. J Mamm Ova Res, 1997, 14:45-48.
[8] Jequier AM, Crich JP.Semen analysis: a practical guide[M]. St. Louis: Blackwell Scientific Publications, 1986.
[9] Byers SL, Payson SJ, Taft RA.Performance of ten inbred mouse strains following assisted reproductive technologies (ARTs)[J]. Theriogenology, 2006, 65(9):1716-1726.
[10] Jishage K, Suzuki H.Maintenance of the Fertilizing Ability in Capacitated Mouse Spermatozoa[J]. J Reprod Develop, 1993, 39(4):363-367.
[11] Takeo T, Tsutsumi A, Omaru T, et al.Establishment of a transport system for mouse epididymal sperm at refrigerated temperatures[J]. Cryobiology, 2012, 65(3):163-168.
[12] Stowe DF, Camara AK, Heisner JS, et al.Ten-hour preservation of guinea pig isolated hearts perfused at low flow with air-saturated Lifor solution at 26{degrees}C: comparison to ViaSpan solution[J]. Am J Physiol Heart Circ Physiol, 2007, 293(1):H895-901.
[13] Stowe DF, Camara AK, Heisner JS, et al.Low-flow perfusion of guinea pig isolated hearts with 26 degrees C air-saturated Lifor solution for 20 hours preserves function and metabolism[J]. J Heart Lung Transplant, 2008, 27(9):1008-1015.

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