The Effect of Carbamazepine on Cardiac Function of Zebrafish Embryo

doi:10.3969/j.issn.1674-5817.2012.06.013

Abstract

Abstract: Objective To explore the feasibility of studying drug toxic and side effect with zebrafish embryos as model. Methods Fourty-eight hours post-fertilization zebrafish embryos were treated with various concentrations of carbamazepine for 24 h. Morphological changes of embryos hearts were observed and heart rate was counted at 8, 16, 24 h after treatment. Then embryos were transferred into embryo medium and the heart rate was observed at 4, 8, 24 h after treatment interruption. Results The heart rate decreased significantly after treatment with carbamazepine for 24 h and showed a good dose-effect relationship with the drug concentration. The 24 h EC₅₀ of decrease of heart rate was about 626.07 µmol/L. Heart rate recovered quickly 8h after treatment interruption.Conclusion Carbamazepine lowered the heart rate of zebrafish embryo in a dose-dependent manner and the repression on cardiac function was reversible.

Key words: Zebrafish, Embryo, Carbamazepine, Heart rate, EC₅₀

CLC Number:

Q95-33

WANG Xue, WANG Xi-min, HAN Li-wen, LIU Ke-chun, HE Qiu-xia, PENG Wei-bing. The Effect of Carbamazepine on Cardiac Function of Zebrafish Embryo[J]. Laboratory Animal and Comparative Medicine, 2012, 32(6): 517-520.

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