Abstract: Objective To establish the method of liver puncture in marmota himalayan. Method The animal was anesthetized by ketamine hydrochloride (45mg/kg) intramuscular injection. Liver puncture point was on the right side of the abdomen in the third and fourth ribs of animals used by biopsy puncture gun, below the sternum xiphoid 1.5 cm, with parallel the midpoint of axillary. Twenty marmota himalayan were punctured with the interval l5days, by total of eighty times from 2009 to 2010. Result Seventy-two liver tissue samples were collected and the success rate was 90%. HE staining confirmed that 70 cases of biopsy tissue had at least three portal areas in the microscope. There were two marmots died caused by liver biopsy and the death rate was 10%. Conclusion Successfully established the liver puncture method of marmota himalayan animal models of hepatitis B, the method was simple, accurate, repeated sampling.

Key words: Liver, Puncture, Marmota himalayana