Laboratory Animal and Comparative Medicine ›› 2011, Vol. 31 ›› Issue (2): 92-95.DOI: 10.3969/j.issn.1674-5817.2011.02.004

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Establishment of Real-time Fluorescent PCR for Rapid Detecting Yersinia Enterocolitica in Monkey

WEN He-xin1, JIANG Rong-hua1, WANG Jie1, SU Dong1, LI Shi-zhao1, PAN Bao-jin2   

  1. 1. Integrated Lab of Guigang Entry-Exit Inspection and Quarantine Bureau, Guigang 537100, China;
    2. Technology Centre of Guangxi Entry-Exit Inspection and Quarantine Bureau, Nanning 530021, China
  • Received:2010-05-12 Online:2011-04-25 Published:2011-04-25

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Abstract: Objective To rapidly detect the Yersinia enterocolitica in monkey ,a real time fluorescent PCR assay was established. Method The primers and probe were designed based on the conserved sequence of heat-labile enterotoxin gene of Yersinia enterocolitica. Then PCR reaction system was optimized and evaluated. Results The established real-time PCR method was sensitive and specific to 3.3×10 cfu/ml bacteria concentration. There was reliable correlation between the template copies (3.3×101 cfu/ml-3.3×107cfu/ml) and the Ct value. Conclusion The real-time PCR assay was proved to be specific with high sensitivity and efficiency, and would be a powerful diagnosis method of Yersinia enterocolitica in monkey.

Key words: Monkey, Yersinia enterocolitica, Real-time PCR, Detection

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