感染日本血吸虫病兔循环抗原的提纯与分析

实验动物与比较医学 ›› 1993, Vol. 13 ›› Issue (3): 125-128.

感染日本血吸虫病兔循环抗原的提纯与分析

1.上海市卫生学校；2.上海医科大学

收稿日期:1992-12-04 出版日期:1993-01-25 发布日期:2013-04-03

Purification and Analysis of the Circulating Antigens of Schistosomiasis Japonica in Rabbits

1.Shanghai Municipal Health School，Shanghai 200237；2.Shanghai Medical University

Received:1992-12-04 Published:1993-01-25 Online:2013-04-03

摘要/Abstract

Abstract: The circulating antigen （CAg） of schistosomiasis japonica from the sera of Schi-stosoma japonicum-infected rabbits was purified by immune-affinity chromatography. The purified CAg exhibited seven protein bands by SDS-PAGE. The molecular weights of them are 144,118, 100，88，63，57 and 46 kD respectively. These CAgs showed five sugar protein bands by PAS colouring. The molecular weights of these sugar proteins are 144,118，100, 63 and 57 kD respectively. The CAgs released from the ova of the schistosome contain 114 and 118 kD sugar proteins as shown by ELIB.

Key words: Schistosomiasis japonica, Circulating antigen, Rabbit

. Purification and Analysis of the Circulating Antigens of Schistosomiasis Japonica in Rabbits[J]. Laboratory Animal and Comparative Medicine, 1993, 13(3): 125-128.