可视化核酸检测技术RPA-LFD的研究和应用进展

doi:10.12300/j.issn.1674-5817.2021.019

摘要/Abstract

摘要： 重组酶聚合酶扩增技术（recombinase ploymerase amplication,RPA）是一种新型恒温核酸扩增技术,与PCR相比,操作简便,反应快速,灵敏度高,特异性强,无需精密仪器,是一种可以替代PCR的核酸检测技术,被认为是目前最有潜力成为快速分子诊断的工具。RPA与横向流动试纸条（lateral flow dipstick, LFD）结合的RPA-LFD技术,可实现扩增产物的可视化检测,在病原体现场核酸快速检测中具有良好的应用前景,这为实验动物的质量监督检验提供了一种新的方法。本文对RPA-LFD这一新型核酸检测方法的技术原理、研究现状、技术瓶颈及用于现场核酸提取的研究进展进行综述。

关键词: 重组酶聚合酶扩增技术, 横向流动试纸条, 现场核酸提取, 动物疫病现场监测

Abstract: Recombinase polymerase amplification (RPA) is a novel isothermal nucleic acid amplification technology. Compared to PCR, it features ease of use, high efficiency, high sensitivity, high specificity, and does not need specific instruments, which allows it as an alternative to PCR and the most promising tool for rapid molecular diagnosis. RPA combined with lateral flow dipstick (LFD) (RPA-LFD) enables visual detection of amplified products and has promising applications for rapid nucleic acid detection of pathogens on site. The novel technique provides a new method for quality supervision and inspection of laboratory animals. In this paper, we reviewed the principle, research status, and technical difficulties of RPA-LFD, as well as the progress in rapid extraction of nucleic acid on site.

Key words: Recombinase polymerase amplification, Lateral flow dipstick, Rapid extraction of nucleic acid on site, Surveillance and control of animal epidemic diseases on site

中图分类号:

Q95-33
R-332

于灵芝,陶凌云,魏晓锋. 可视化核酸检测技术RPA-LFD的研究和应用进展[J]. 实验动物与比较医学, 2021, 41(6): 547-553. DOI: 10.12300/j.issn.1674-5817.2021.019.

YU Lingzhi,TAO Lingyun,WEI Xiaofeng. Research and Application Progress in Visualized RPA-LFD Nucleic Acid Detection Technology[J]. Laboratory Animal and Comparative Medicine, 2021, 41(6): 547-553. DOI: 10.12300/j.issn.1674-5817.2021.019.

图/表 1

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