实验动物与比较医学 ›› 2012, Vol. 32 ›› Issue (5): 421-425.DOI: 10.3969/j.issn.1674-5817.2012.05.011

• 论著 • 上一篇    下一篇

番茄红素对糖尿病大鼠肾组织血管紧张素转换酶2表达的影响

吴博1, 江海燕2, 王一龙1, 陈通克1, 朱加银1, 赵惠玲1   

  1. 1.温州医学院 实验动物中心;
    2.眼视光学院, 温州 325035
  • 收稿日期:2012-03-28 出版日期:2012-10-25 发布日期:2012-10-25
  • 作者简介:吴博(1985-),男,助理实验师,硕士研究生,主要从事实验动物和比较医学研究,E-mail:wubo_0611@163.com;共同第一作者:江海燕(1985-),女,助理实验师,硕士研究生,主要从事糖尿病肾病发病机理及治疗研究,E-mail:pharmacy000@163.com
  • 基金资助:
    浙江省实验动物科技计划项目(2009F80024)

Effect of Lycopene on Angiotensin-converting Enzyme 2 Expression in Kidney Tissue of Rats with Diabetic Nephropathy

WU Bo1, JIANG Hai-yan2, WANG Yi-long1, CHEN Tong-ke1, ZHU Jia-yin1, ZHAO Hui-ling1   

  1. 1. Center of Laboratory Animal;
    2. School of Optometry and Ophthalmology,Wenzhou Medical College, Wenzhou 325035, China
  • Received:2012-03-28 Online:2012-10-25 Published:2012-10-25

摘要: 目的 通过观察糖尿病大鼠肾组织血管紧张素转换酶2(ACE2)的表达变化,探讨番茄红素的肾保护作用机制。方法 实验设5组: 正常对照组(NC组)、糖尿病模型对照组(DM组)、番茄红素高剂量组(20 mg·mg-1·d-1)、中剂量组(10 mg·mg-1·d-1)和低剂量组(5 mg·mg-1·d-1)。8 周后,测定大鼠血液和尿液相关指标, 免疫组织化学和Western blotting法检测肾组织ACE2蛋白的表达。结果 DM组和番茄红素治疗组的血糖、胰岛素、血肌酐、尿素氮、血管紧张素Ⅱ及24 h尿蛋白均显著高于NC组(P<0.05); 经番茄红素治疗后,血肌酐、尿素氮、血管紧张素Ⅱ和24 h尿蛋白均显著改善(P<0.05); 与NC组相比,DM组的ACE2阳性表达和蛋白表达水平均显著降低(P<0.01),而番茄红素高、中剂量组的ACE2阳性表达和蛋白表达水平则均显著高于DM组(P<0.05),其中高剂量效果更为显著(P<0.01)。结论 番茄红素能降低糖尿病肾病(DN)大鼠尿蛋白、改善肾功能及提高肾组织ACE2蛋白的表达。

关键词: 番茄红素, 糖尿病肾病, 血管紧张素转化酶2

Abstract: Objective To observe the expression changes of angiotensin-converting enzyme 2(ACE2) in kidney tissue of rats with diabetic nephropathy, and explore the renal protection mechanism of lycopene. Methods The rats were divided into 5 groups: nomal control group, diabetic nephropathy (DM) model group, and Lycopene treated groups with high-dose (20 mg·kg-1·d-1), middle-dose (10 mg·kg-1·d-1), and low-dose (5 mg·kg-1·d-1) respectively. After 8 weeks, the blood and urine index was measured. The expression level of ACE2 protein in kidney tissue was detected respectively by immunohistochemistry and western blotting. Results The concentrations of blood glucose, serum insulin, creatinine, urea nitrogen, AngII and urine protein in lycopene and diabetes groups were all significantly higher than those of normal control group (P<0.05); After treated by lycopene, serum creatinine, urea nitrogen, AngII and urine protein were greatly improved (P<0.05). The expression of ACE2 protein in diabetes group was greatly decreased than that of normal control group (P<0.01), while ACE2 protein levels in the high- and middle-dose groups treated with lycopene were obviously up-regulated (P<0.05), especially in high-dose lycopene group (P<0.01). Conclusion Lycopene can decrease urine protein, improve the renal function, and up-regulate the expression of ACE2 protein in diabetic nephropathy.

Key words: Lycopene, Diabetic nephropathy, ACE2

中图分类号: