Poly I:C刺激对裸鼹鼠体内自噬水平的影响

doi:10.3969/j.issn.1674-5817.2014.05.014

摘要/Abstract

摘要： 目的研究多聚肌苷-多聚胞苷酸钠盐(Poly I:C)对裸鼹鼠自噬水平的影响。方法成年裸鼹鼠以及C57BL/6小鼠分别随机分成Poly I:C给药组和对照组,分别腹腔注射PolyI:C和生理盐水。Poly I:C给药12 h后,组织切片HE染色观察小肠组织的病理变化,电镜观察细胞结构的变化,实时定量PCR检测小肠组织中炎症相关因子IL-1、ATG5的mRNA表达情况,蛋白印迹检测小肠组织中自噬相关蛋白LC3B、Beclin 1表达情况。结果 Poly I:C给药后,裸鼹鼠小肠组织未出现显著的病理变化,但C57BL/6小鼠小肠组织腺体部出现广泛性出血;电镜观察发现,裸鼹鼠小肠组织细胞中线粒体增多、变长,自噬体数量显著增加,而小鼠组织细胞线粒体有大量的空泡化,滑面内质网扩张; 实时定量PCR检测未发现组织中IL-1、ATG5 mRNA表达有显著变化;蛋白印迹检测发现,给药后裸鼹鼠组织中LC3B蛋白表达显著上调(P<0.05),而C57BL/6小鼠则下调(P<0.05)。结论 Poly I:C给药引起裸鼹鼠自噬水平上调,同时,裸鼹鼠自噬水平的上调亦可以提高机体的适应能力,进而起到抵抗Poly I:C损伤的作用。

关键词: 裸鼹鼠, 多聚肌苷-多聚胞苷酸钠盐(Poly I:C), 炎症, 自噬

Abstract: Objective To study the effect of polyinosinic-polycytidylic acid(Poly I: C) on the autophagy in naked mole rat. Methods The adult naked mole rats and C57BL/6 mice were randomly divided into Poly I: C treatment group (ip Poly I: C) and control group (ip saline). Twelve hours after Poly I: C administered, the differences in the intestinal pathological changes and subcellular organelles between naked mole rats and C57BL / 6 mice were observed by light and electron microscopy respectively. The mRNA expression of inflammation-related factor IL-1 and autophagy-related gene ATG5 in intestinal tissue were detected by quantitative real-time PCR. Detection of autophagy-related proteins LC3B and Beclin 1 was conducted by Western blot. Results Naked mole rats did not show significant pathological changes after Poly I: C treatment, but intestinal tissue glands of C57BL/6 mouse show serious hemorrhage. Further observation by electron microscope found that the mitochondria’s size was elongagted, number increased and more autophagy in naked mole rats’ intestinal tissue cells. Meanwhile, a lot of vacuolatation of mitochondria, dilation of smooth endoplasmic reticulum was observed in mouse intestinal tissues. Significant difference was not found in mRNA expression of IL-1, ATG5 by real-time quantitative PCR. In Western blot detection, LC3B protein expression was significantly increased (P<0.05) in naked mole rats, after administration, but that of C57BL/6 mice were reduced (P<0.05). Conclusion Poly I: C administration induced upregulation of autophagy in naked mole rat. While, autophagy may regulate the inflammatory response in turn, which played an important role in naked mole rats’ anti-Poly I:C injury.

Key words: Naked mole rat, polyinosinic-polycytidylic acid(Poly I: C), Inflammation, Autophagy

中图分类号:

Q95-33

林丽芳, 肖邦, 赵善民, 汤球, 余琛琳, 孙伟, 张璐, 蔡丽萍, 徐晨, 程继帅, 崔淑芳. Poly I:C刺激对裸鼹鼠体内自噬水平的影响[J]. 实验动物与比较医学, 2014, 34(5): 411-416.

LIN Li-fang, XIAO Bang, ZHAO Shan-min, TANG Qiu, YU Chen-lin, SUN Wei, ZHANG Lu, CAI Li-ping, XU Chen, CHENG Ji-shuai, CUI Shu-fang. The Effect of Poly I:C on Autophagy of Naked Mole Rat[J]. Laboratory Animal and Comparative Medicine, 2014, 34(5): 411-416.

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