关键词: 小鼠剪尾出血模型, 创伤模拟, 出血量, 出血时间

Abstract: Objective The application of tail tip bleeding model in mice was explored to evaluate the hemostatic activity of some coagulation components. Method This tail tip bleeding model improved to be used as a trauma model in the aspect of coagulant evaluation was compared with the traditional operation process. The new method was established for fast quantitative detection of the blood loss and decided the bleeding time from the starting point when the tail was cut off to the end point when the blood loss was less than 1 μL in two minutes intervals. Saculin approved as a coagulant drug on sale in domestic market was used to verify the effectiveness of this assay. The sensitivity between coagulate time and blood loss (or bleeding time) is also compared in mice. Results The advanced tail tip bleeding model in mice can quantitative analysis blood loss and bleeding time in order to reduce the personal error to a great extent. This model is easy to use and can simulate the process of trauma properly. Conclusion The tail tip bleeding model in mice can reflect the action of the coagulant drug sensitively, which is a useful tool for screening and assessment of coagulants.

Key words: Tail tip bleeding model, Mice, Simulated trauma, Blood loss, Bleeding time