封闭群树鼩的微卫星遗传特性分析

doi:10.3969/j.issn.1674-5817.2018.01.001

实验动物与比较医学 ›› 2018, Vol. 38 ›› Issue (1): 1-9.DOI: 10.3969/j.issn.1674-5817.2018.01.001

所属专题：实验动物资源开发与利用

• 论著 • 下一篇

封闭群树鼩的微卫星遗传特性分析

刘城秀, 李娜, 仝品芬, 王文广, 陆彩霞, 罕园园, 匡德宣, 孙晓梅, 代解杰

中国医学科学院/北京协和医学院医学生物学研究所,云南省重大传染病疫苗研发重点实验室,昆明 650118

收稿日期:2017-09-07 出版日期:2018-02-25 发布日期:2021-03-01
作者简介:刘城秀(1986-),女,博士研究生,研究方向:实验动物遗传学。E-mail:liu.chengxiu@163.com
基金资助:
国家科技支撑计划项目(2014BAI01B00),云南省科技人才和平台计划项目(2017HC019),云南省重大科技专项(2017ZF007)

Analysis of Microsatellite Genetic Characteristics in of Closed Colony of Tree Shrews

LIU Cheng-xiu, LI Na, TONG Pin-fen, WANG Wen-guang, LU Cai-xia, HAN Yuan-yuan, KUANG De-xuan, SUN Xiao-mei, DAI Jie-jie

The Institute of Medical Biology,Chinese Academy of Medical Science & Peking Union Medical College,Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases,Kunming 650118,China

Received:2017-09-07 Online:2018-02-25 Published:2021-03-01

摘要/Abstract

摘要： 目的比较分析远交繁殖的I~IV代次的滇西亚种树鼩群体的遗传差异和分化程度,为培育封闭群树鼩种群提供遗传学依据。方法提取I~IV四个代次共49只滇西亚种树鼩的全血基因组DNA,选取树鼩的18个微卫星位点,结合荧光标记(FAM) PCR扩增技术,通过毛细管电泳技术检测扩增产物,并利用POPGENE、FSTAT等软件比较各代次树鼩的遗传相关指标。结果四个代次的树鼩群体共检测到等位基因(Na)89个,平均有效等位基因数(Ne)为3.779,平均观察杂合度(Ho)为0.523,平均期望杂合度(He)为0.613,平均多态信息含量(PIC)为0.558,平均Shannon信息指数(I)为1.277,平均等位基因丰富度(AR)为2.938,遗传分化系数(Fst)平均值为0.046;其遗传距离及无偏遗传距离分别为0.049~0.159和0.022~0.109。结论培育的树鼩群体的遗传多样性较丰富,且不存在较大的遗传差异和分化程度。

关键词: 滇西亚种树鼩, 封闭群, 微卫星位点, 遗传多样性, 遗传管理

Abstract: Objective To find out the genetic variation and differentiation of the I~IV generations in tree shrews (Tupaia belangeri chinensis),and provide genetic bases for closed colony tree shrews.Methods The forty-nine genomic DNA of blood samples were extracted from I~IV generations tree shrews,the amplification products were detected by capillary electrophoresis and marked with fluorescent tags (FAM) PCR,then the genetic diversity of the tree shrew colony was analyzed comparatively using POPGENE,FSTAT software with tree shrews 18 microsatellite loci.Results The 89 alleles were found in the tree shrew colony,the average effective number of alleles,observed heterozygosity,expected heterozygosity,polymorphism information content,Shannon information index and allele richness were 3.779,0.523,0.613,0.558,1.277 and 2.938 respectively.The mean value of Fst was 0.046 in the colony.The genetic distance and unbiased genetic distance were 0.049~0.159 and 0.022~0.109 respectively.Conclusions The closed clony tree shrew shows high genetic diversity,and there was no significant difference in genetic diversity and differentiation.

Key words: Tree shrew (Tupaia belangeri chinensis), Closed colony, Microsatellite loci, Genetic diversity, Genetic management

中图分类号:

Q95-33

刘城秀, 李娜, 仝品芬, 王文广, 陆彩霞, 罕园园, 匡德宣, 孙晓梅, 代解杰. 封闭群树鼩的微卫星遗传特性分析[J]. 实验动物与比较医学, 2018, 38(1): 1-9.

LIU Cheng-xiu, LI Na, TONG Pin-fen, WANG Wen-guang, LU Cai-xia, HAN Yuan-yuan, KUANG De-xuan, SUN Xiao-mei, DAI Jie-jie. Analysis of Microsatellite Genetic Characteristics in of Closed Colony of Tree Shrews[J]. Laboratory Animal and Comparative Medicine, 2018, 38(1): 1-9.

参考文献

[1] Fan Y,Huang ZY,Cao CC,et al.Genome of the Chinese tree shrew[J].Nat Commun,2013,4:1426.
[2] 许凌,范宇,蒋学龙,等.树鼩进化分类地位的分子证据[J].动物学研究,2013,34(2):70-76.
[3] Yu W,Yang C,Bi Y,et al.Characterization of hepatitis E virus infection in tree shrew (Tupaia belangeri chinensis)[J].BMC Infect Dis,2016,16:80.
[4] Lu JS,Yue F,Liu X,et al.Characterization of the anterior cingulate cortex in adult tree shrew[J].Mol Pain,2016,12:1-11.
[5] Pryce CR,Fuchs E.Chronic psychosocial stressors in adulthood:Studies in mice,rats and tree shrews[J].Neurobiol Stress,2017,6:94-103.
[6] Gawne TJ,Siegwart JT,Ward AH,et al.The wavelength composition and temporal modulation of ambient lighting strongly affect refractive development in young tree shrews[J].Exp Eye Res,2017,155:75-84.
[7] Chen SY,Xu L,Lu LB,et al.Genetic diversity and matrilineal structure in Chinese tree shrews inhabiting Kunming,China[J].Dongwuxue Yanjiu,2011,32(1):17-23.
[8] Souza CA,Paiva SR,Mcmanus CM,et al.Genetic diversity and assessment of 23 microsatellite markers for parentage testing of Santa Ines hair sheep in Brazil[J].Genet Mol Res,2012,11(2):1217-1229.
[9] Nachtigall M,Bulow L,Schubert J,et al.Development of SSR markers for the genus Patellifolia (Chenopodiaceae) [J].Appl Plant Sci,2016,4(8).pⅡ:apps.1600040.
[10] 黎家敏,李海燕,李婧潇,等.应用RAPD标记技术对树鼩遗传多样性的分析[J].中国比较医学杂志,2010,20(2):34-40.
[11] 张媛,李晓飞,李振宇,等.滇西亚种树鼩微卫星分子标记的筛选[J].中国比较医学杂志,2015,25(6):36-41.
[12] Srikwan S,Hufford K,Eggert L,et al.Variable microsatellite markers for genotyping tree shrews,tupaia,and their potential use in genetic studies of gragmented populations[J].Science Asia,2002,28(28):93-97.
[13] Liu XH,Yao YG.Characterization of 12 polymorphic microsatellite markers in the Chinese tree shrew (Tupaia belangeri chinensis)[J].Dongwuxue Yanjiu,2013,34(E2):E62-68.
[14] Munshi-South J,Wilkinson GS.Isolation and characterization of polymorphic microsatellite loci in Bornean tree shrews (Tupaia spp.)[J].Molecular Ecology Notes,2006,6(3):698-699.
[15] Glaubitz JC.convert:A user-friendly program to reformat diploid genotypic data for commonly used population genetic software packages[J].Mol Ecol Resour,2004,4(2):309-310.
[16] Raymond M,Rousset F.GENEPOP (version1.2):population genetics software for exact tests and ecumenicism[J].J Heredity,1995,86(3):248-249.
[17] Rousset F.DENEPOP'007:a complete re-implementation of the genepop software for Windows and Linux[J].Mol Ecol Resour,2008,8(1):103-106.
[18] Su AL,Lan XW,Huang MB,et al.Microsatellite analysis of genetic diversity in the Tupaia belangeri yaoshanensis[J].Biomedical Reports,2017,7(4):349-352.
[19] Botstein D,White RL,Skolnick M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].Am J Hum Genet,1980,32(3):314-331.
[20] 李薇,江其辉,杜小燕,等.封闭群长爪沙鼠遗传标准的建立[J].实验动物科学,2011,28(2):31-36.
[21] 唐艳萍,曹骥,杨香娣,等.广西和云南树鼩群体遗传多样性的比较分析[J].中国实验动物学报,2016,24(6):572-578.
[22] 杨香娣,曹骥,杨春,等.荧光标记微卫星分析广西树鼩群体的遗传多态性[J].中国畜牧兽医,2016,43(1):182-190.
[23] 杨芳,何保丽,何永蜀,等.树鼩5个生化位点与11个微卫星标记位点的初步研究[J].昆明医学院学报,2010,31(5):8-11+16.
[24] 张媛.滇西亚种树鼩微卫星筛选及群体多态性分析[D].北京:北京协和医学院,2015.
[25] 王琳琳,徐金会,秦桢,等.三个微卫星标记在喜马拉雅旱獭种群中的遗传多态性[J].曲阜师范大学学报:自然科学版,2008,34(4):101-104.

封闭群树鼩的微卫星遗传特性分析

Analysis of Microsatellite Genetic Characteristics in of Closed Colony of Tree Shrews

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