脂多糖对裸鼹鼠肺脏组织影响的初探

doi:10.3969/j.issn.1674-5817.2020.06.010

实验动物与比较医学 ›› 2020, Vol. 40 ›› Issue (6): 523-527.DOI: 10.3969/j.issn.1674-5817.2020.06.010

脂多糖对裸鼹鼠肺脏组织影响的初探

张成财^1*, 刘尹航^2*, 陈超¹, 江文正³

1.海军军医大学基础医学院实验动物学教研室,上海 200433;
2.上海外国语大学附属中学,上海 200083;
3.华东师范大学生命科学学院,上海 200241

收稿日期:2020-10-10 出版日期:2020-12-25 发布日期:2020-12-30
作者简介:张成财(1992-), 男, 硕士研究生。研究方向: 动物学。E-mail: zhangchengcai_06@163.com
基金资助:
[基金项目]上海市“科技创新行动计划”实验动物研究领域项目(20140900100)

A Preliminary Study on Effects of Lipopolysaccharide on Lung Tissues of Naked Mole Rats

ZHANG Chengcai^1*, LIU Yinhang^2*, CHEN Chao¹, JIANG Wenzheng³

1. Department of Laboratory Animal Science, School of Basic Medical Sciences,Naval Medical University, Shanghai 200433, China;
2. Shanghai Foreign Language School,Shanghai 200083, China;
3. School of Life Sciences, East China Normal University, Shanghai 200241

Received:2020-10-10 Online:2020-12-25 Published:2020-12-30

摘要/Abstract

摘要： 目的观察和分析脂多糖（lipopolysaccharide,LPS）对裸鼹鼠肺脏组织形态结构和病理学相关指标的影响,初步探讨裸鼹鼠是否存在LPS耐受。方法雄性裸鼹鼠10只,随机分为实验组和对照组。实验组按体质量以10 mg/kg给药量腹腔注射LPS,对照组注射同等体积的0.9%NaCl溶液（生理盐水）。给药后6 h,采集肺脏,检测肺组织湿干重量比值;制作石蜡切片,经HE染色后,光学显微镜下观察肺组织显微结构; 流式细胞仪检测肺组织细胞中活性氧含量、肺组织细胞凋亡率。结果实验组裸鼹鼠肺组织结构完整,肺泡孔内无明显渗出液和炎性细胞浸润等病变。与对照组比较,实验组肺组织湿干质量比未发生显著变化,早期凋亡率未发生显著变化,但晚期凋亡率显著升高（P <0.01）,活性氧含量显著升高（P < 0.05）。结论裸鼹鼠对LPS诱导的肺损伤有一定程度的耐受性,其具体机制有待进一步研究。

关键词: 裸鼹鼠, 肺脏, 脂多糖

Abstract: Objective To detect the effects of lipopolysaccharide (LPS) on lung morphology and pathological parameters, and explore the LPS tolerance in naked mole rats. Methods Ten male naked mole rats were randomly divided into an experimental group and a control group. The naked mole rats of the experimental group were injected with LPS at 10 mg/kg according the body weight, while the naked mole rats of the control group were injected with the same volume of normal saline. Six hours after the administration, lung tissues were collected to detect the ratio of wet to dry weight; paraffin sections were prepared and stained with HE, and the lung microstructure was observed under optical microscope; the level of reactive oxygen species (ROS) and the apoptosis ratio of lung tissue cells were detected by flow cytometry. Results Microscopic examination showed that the lung tissue structure of naked mole rats in the experimental group was intact, and there were no obvious exudate and inflammatory cell infiltration in the alveolar foramen. Compared with the control group, the ratio of wet to dry weight of lung tissues in the experimental group and the proportion of early apoptosis had no significant differences, but the proportion of late apoptosis and the content of ROS increased significantly (P < 0.01, and P < 0.05, respectively). Conclusion Naked mole rats can tolerate the lung injury induced by LPS to some degree, and the specific mechanism needs to be further studied.

Key words: Naked mole rats, Lung, Lipopolysaccharide

中图分类号:

Q95-33

张成财, 刘尹航, 陈超, 江文正. 脂多糖对裸鼹鼠肺脏组织影响的初探[J]. 实验动物与比较医学, 2020, 40(6): 523-527.

ZHANG Chengcai, LIU Yinhang, CHEN Chao, JIANG Wenzheng. A Preliminary Study on Effects of Lipopolysaccharide on Lung Tissues of Naked Mole Rats[J]. Laboratory Animal and Comparative Medicine, 2020, 40(6): 523-527.

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参考文献

[1] Jarvis JU.Eusoeiality in a mammal: cooperative breeding in naked mole-rat colonies[J]. Science, 1981, 212(4494):571-573. doi: 10.1126/science.7209555.
[2] Sherman PW, Jennifer J, Richard A.The Biology of the Naked Mole-rat[M]. Princeton: Princeton University Press, 1992:433-434.
[3] 赵善民, 崔淑芳. 裸鼹鼠生物学特性的研究进展[J]. 实验动物与比较医学, 2013, 33(5):400-405.
[4] 马晓春, 王辰, 方强, 等. 急性肺损伤/急性呼吸窘迫综合征诊断和治疗指南[J]. 中国危重病急救医学, 2006, 18(12):706-710.
[5] Matthay MA, Zimmerman GA, Esmon C, et al.Future research directions in acute lung injury: summary of a National Heart, Lung, and Blood Institute working group[J]. Am J Respir Crit Care Med, 2003, 167(7):1027-1035. doi: 10.1164/rccm.200208-966WS.
[6] Ware LB, Matthay MA.The acute respiratory distress syndrome[J]. N Engl J Med, 2000, 342(18):1334-1349. doi: 10.1056/NEJM200005043421806.
[7] Brower RG, Matthay MA, Morris A, et al.Ventilation with lower tidal volumes as compared with traditional tidal volumes for acute lung injury and the acute respiratory distress syndrome[J]. N Engl J Med, 2000, 342(18):1301-1308. doi: 10.1056/NEJM200005043421801.
[8] Krafft P, Fridrich P, Pernerstorfer T, et al.The acute respiratory distress syndrome: definitions, severity and clinical outcome. An analysis of 101 clinical investigations[J]. Intensive Care Med, 1996, 22(6):519-529. doi: 10.1007/BF01708091.
[9] Lu Y, Song Z, Zhou X, et al.A 12-month clinical survey of incidence and outcome of acute respiratory distress syndrome in Shanghai intensive care units[J]. Intensive Care Med, 2004, 30(12):2197-2203. doi: 10.1007/s00134-004-2479-y.
[10] Atabai K, Matthay MA.The pulmonary physician in critical care·5: Acute lung injury and the acute respiratory distress syndrome: definitions and epidemiology[J]. Thorax, 2002, 57(5):452-458. doi: 10.1136/thorax.57.5.452.
[11] Sessler CN, Bloomfield GL, Fowler AA 3rd. Current concepts of sepsis and acute lung injury[J]. Clin Chest Med, 1996, 17(2):213-235. doi: 10.1016/s0272-5231(05)70310-5.
[12] 杨敏华, 姚友杰, 王娟. 姜油酮通过激活Nrf2通路减轻LPS诱导的小鼠急性肺损伤[J]. 西安交通大学学报, 2020, 41(1):150-156.
[13] Fanelli V, Vlachou A, Ghannadian S, et al.Acute respiratory distress syndrome: new definition, current and future therapeutic options[J]. J Thorac Dis, 2013, 5(3):326-334. doi: 10.3978/j.issn.2072-1439.2013.04.05.
[14] Matthay MA, Zemans RL.The acute respiratory distress syndrome: pathogenesis and treatment[J]. Annu Rev Pathol, 2011, 6:147-163. doi: 10.1146/annurev-pathol-011110-130158.
[15] Capelozzi VL.What have anatomic and pathologic studies taught us about acute lung injury and acute respiratory distress syndrome[J]. Curr Opin Crit Care, 2008, 14(1):56-63. doi: 10.1097/MCC.0b013e3282f449de.
[16] Cross LJ, Matthay MA.Biomarkers in acute lung injury: insights into the pathogenesis of acute lung injury[J]. Critical care clinics, 2011, 27(2):355-377. doi: 10.1016/j.ccc.2010.12.005.
[17] Victor VM, Rocha M, De-la-Fuente M. Immune cells: free radicals and antioxidants in sepsis[J]. Int Immunopharmacol, 2004, 4(3):327-347. doi: 10.1016/j.intimp.2004.01.020.
[18] 孙伟, 汤球, 赵善民, 等. 裸鼹鼠的人工饲养繁育初步研究[J]. 实验动物与比较医学, 2013, 33(4):296-300.
[19] Larson J, Park TJ.Extreme hypoxia tolerance of naked mole-rat brain[J]. Neuroreport, 2009, 20(18):1634-1637. doi: 10.1097/WNR.0b013e32833370cf.

脂多糖对裸鼹鼠肺脏组织影响的初探

A Preliminary Study on Effects of Lipopolysaccharide on Lung Tissues of Naked Mole Rats

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