侧脑室注射μ-阿片受体反义寡聚核苷酸对小鼠甲基苯丙胺行为敏化反应的影响

›› 2007, Vol. 27 ›› Issue (3): 155-159.

侧脑室注射μ-阿片受体反义寡聚核苷酸对小鼠甲基苯丙胺行为敏化反应的影响

1.苏州大学医学院基础医学系生理学教研室，苏州 215123；2.密西西比大学医学研究中心药理与毒理学研究室，美国密西西比 39216

收稿日期:2007-04-16 出版日期:2007-03-31 发布日期:2007-03-31

Effect of μ-Opioid Receptor Antisense Oligodeoxynucleotide on Methamphetamine-Induced Behavioral Sensitization in Mice

1 .Department of Physiology, Medical School of Soochow University, Suzhou 215123, China),2. Department of Pharmacology and Toxicology, University of Mississippi Medical Center, MS 39216 ,U.S.A

Received:2007-04-16 Online:2007-03-31 Published:2007-03-31

摘要/Abstract

摘要： 目的探讨μ-阿片受体（μ-opioid receptor，MOR）在小鼠甲基苯丙胺行为敏化中的作用.方法人工合成反义及错配反义MOR基因片断，通过对小鼠自主活动行为的检测，观测侧脑室注射MOR反义寡聚核苷酸（Antisense oligodeoxynucleotide，ASODN）对急性甲基苯丙胺活动增强效应及慢性甲基苯丙胺诱导的小鼠行为敏化形成的影响.结果 MORASODN可显著降低甲基苯丙胺的急性高活动效应，并可阻断甲基苯丙胺诱导小鼠行为敏化的形成.结论 MORASODN对中枢神经系统功能具有抑制作用，可抑制甲基苯丙胺诱导小鼠行为敏化的形成过程，提示MOR介导对甲基苯丙胺的成瘾行为的调控作用.

关键词: μ-阿片受体, 反义寡聚核苷酸, 甲基苯丙胺, 行为敏化

Abstract: Objective To investigate the effect of u-opioid receptor (MOR) on the process of methamphetamine (METH) - induced behavioral sensitization in mice. Methods The antisense and mismatch oligodeoxynucleotides(ODN) for MOR gene were synthesized and delivered individually to mice by intracerebroventricular injection (i.c.v.). Locomotor activity was detected after acute administration with METH in mice. Effects of repeated intermittent administrations of METH on locomotor activity were measured to observe the effects of MOR on the development of behavioral sensitization to METH. Results MOR ASODN could attenuate the METH-induced hyperlocomotor activity in mice and MOR ASODN-exposed mice failed to sensitize to the METH challenge. Conclusion MOR may play an important neuromodulatory role in METH- induced behavior sensitization.

Key words: u-opioid receptor, Anti sense oligodeoxynucleotide, Methamphetamine, Behavioral sensitization

沈新娥1，王国卿1，周希平1，邹容1，马谭庚2. 侧脑室注射μ-阿片受体反义寡聚核苷酸对小鼠甲基苯丙胺行为敏化反应的影响[J]. , 2007, 27(3): 155-159.

SHEN Xin-e1,WANG Guo-qing1,ZHOU Xi-ping1,ZOU Rong1,MA Tan-geng2. Effect of μ-Opioid Receptor Antisense Oligodeoxynucleotide on Methamphetamine-Induced Behavioral Sensitization in Mice[J]. , 2007, 27(3): 155-159.