›› 1991, Vol. 11 ›› Issue (1): 16-18.

• 论文 • 上一篇    下一篇

猕猴体外授精研究初报

  

  1. 华南濒危动物研究所
  • 收稿日期:1989-01-09 出版日期:1991-01-25 发布日期:2013-03-21

"A PRELIMINARY STUDY ON FERTILIZATION OF RHESUS MONKEY IN VITRO "

  1. South China istitute of Endangered Animals
  • Received:1989-01-09 Online:1991-01-25 Published:2013-03-21

摘要: 1986年10~12月,对10头人工饲养的成年雌猴,采用外源性激素作超数排卵及体外授精实验。以4种方法进行超排处理,外科手术采卵。采卵总数49个,x=4.9±4.58。均在5%CO237℃的培养箱培养成熟后,进行体外授精。在采卵前4~6小时,对雄猴直肠电刺激采得精液。其中一例在授精35小时后观察:8个卵细胞中,有一个出现雌雄原核,另一个已发育到16个细胞以上的桑椹期胚胎。

Abstract: "The experiment was carried out from October to December, 1986. 10 adult female rhesus monkeys were divided into five groups and each group includes two monkeys. The monkeys of group B and E had normal menstrual cycles. The menstrual cycles of group A and C were not in consideration and the menstrual cyclcs of group D were induced artificially by 50 mg progesterone and 4 mg PGF 2 a. All monkeys were superstimulated by daily injection of gonadotrophin hormones. Four superstimulated methods were performed. 49 oocytes were collected by the surgical operation in 19~36 hours after hCG treatment. The semen was collected by elcctric-ejaculation 4~6 hours before oocytes collection. The oocytes and semen were prcincubated and fertilized in F-10 medium at 37℃ in a humidified incubutor under 5% CO2 in air. 2 of 8 oocytes showed morphological signs consistent with fertilization 35 h later: a two pronucleus embryo and a morula-staged embryo. "

Key words: Rhesus monkey, Fertilization, Embryo.