人乙肝病毒转基因小鼠肝脏形态观察和DNA图象分析初报

实验动物与比较医学 ›› 1995, Vol. 15 ›› Issue (4): 206-209.

人乙肝病毒转基因小鼠肝脏形态观察和DNA图象分析初报

1.上海市肿瘤研究所；2.杨州大学农学院

收稿日期:1995-05-30 出版日期:1995-01-25 发布日期:2013-03-19
基金资助:
“八五”攻关基金

Histopathological Observation and DNA Image Analysis on Livers of HBV Transgenic Mice

Shanghai Cancer Institute,Shanghai 200032

Received:1995-05-30 Published:1995-01-25 Online:2013-03-19

摘要/Abstract

摘要： 观察了32例23～55日龄人乙肝病毒转基因小鼠的肝脏形态，发现17例小鼠的部分肝细胞巨大，核呈圆形、椭圆形或不规则形，核膜厚，核染色质粗大、浓染，核仁大而明显，核质比例大，肝小叶结构基本完整。其中的7例异常肝细胞所占比例较高，但未见典型的肝炎病变。DNA图象分析显示，异常细胞核内DNA含量增加，DNA含量大于五倍体的细胞占各期细胞总数的比例达12%～56%。

关键词: 乙肝病毒转基因小鼠, DNA图象分析, 异常肝细胞

Abstract: "The histopathological observation on the livers of thirty two ICR/JCL HBV transgenic mice at 23—55 days old revealed that in 17 mice there were enlarged hepatocytes with round, oval or irregularly shaped nucleus, thick nuclear membrane, coarse and dark-stained chromatin, enlarged nucleoli and increased nucleus-cytoplasmic ratio. The structure of liver lobules was essentially normal. The proportion of such abnormal cells was relatively high in 7 of the 17 mice, but there were no typical pathological changes of hepatitis in these cases. Analysis of DNA images showed that there was an increase in DNA content of the abnormal hepatocytic nuclei. The proportion of the cells with the number of chromosomes over penta-ploid reached 12%—56%. "

Key words: "HBV transgenic mouse, DNA image, Histopathology "

万曙光1,励雁峰1,张萍萍1,等. 人乙肝病毒转基因小鼠肝脏形态观察和DNA图象分析初报[J]. 实验动物与比较医学, 1995, 15(4): 206-209.

WAN Shu-Guang-1,LI Yan-Feng-1,ZHANG Ping-Ping-1,et al. Histopathological Observation and DNA Image Analysis on Livers of HBV Transgenic Mice[J]. Laboratory Animal and Comparative Medicine, 1995, 15(4): 206-209.