上海交通大学实验动物中心公共细胞培养平台管理经验探讨

doi:10.12300/j.issn.1674-5817.2023.101

摘要/Abstract

摘要：

公共细胞培养平台作为实验动物设施中一个重要的配套设施，是实验动物肿瘤疾病模型以及转基因动物制备等科学研究的重要支撑。实验动物中心搭建公共细胞培养实验平台，能充分整合实验动物和细胞培养资源，更为高效、合理地配置相关科研资源，便于科研人员更好地开展研究。实验动物中心公共细胞培养平台内培养的细胞大部分用于动物实验，细胞如果存在污染或者质量问题，不但影响实验结果，还会影响实验动物健康，造成实验动物的微生物感染，严重者甚至会扩散污染整个动物饲养设施。因此实验动物中心的公共细胞培养实验室在细胞质量把控上相比常规的细胞培养室更为严格。本文以上海交通大学实验动物中心公共细胞培养平台为例，从设施维护与管理、人员管理、细胞生物风险质控管理等三个主要控制点对公共细胞培养室的管理经验进行了探讨，以期为国内其他实验动物机构的公共细胞培养实验室管理提供有益借鉴。

关键词: 细胞培养室, 设施维护与管理, 人员管理, 细胞生物风险质控管理

Abstract:

Public cell culture platform is an important facility in laboratory animal facilities, providing essential support for scientific research such as the development of animal tumor disease models and transgenic animals. By establishing a public cell culture experimental platform, laboratory animal centers can effectively integrate experimental animals and cell culture resources, optimizing the allocation of scientific research resources to facilitate better research outcomes. The majority of cells cultured in these platforms are used for animal experiments. Contamination or quality issues in these cells not only affect experimental results but also jeopardize the health of experimental animals, potentially leading to microbial infections and contamination of entire animal facilities. Therefore, public cell culture laboratories within experimental animal facilities impose stricter quality control measures than conventional cell culture rooms. This study takes the public cell culture platform at the Laboratory Animal Center of Shanghai Jiao Tong University as a case study to discuss management experiences, focusing on facility maintenance and management, personnel management and quality control of cell biological risk. The aim is to provide useful reference for the management of public cell culture laboratories in experimental animal facilities and other institutions.

Key words: Cell culture laboratory, Facility maintenance and management, Personnel management, Cell biological risk quality control management

中图分类号:

Q95-33

彭丽娜,张曼,艾敏,等. 上海交通大学实验动物中心公共细胞培养平台管理经验探讨[J]. 实验动物与比较医学, 2024, 44(2): 227-233. DOI: 10.12300/j.issn.1674-5817.2023.101.

Lina PENG,Man ZHANG,Min AI,et al. Discussion on Management Experience of Public Cell Culture Platform in Laboratory Animal Center of Shanghai Jiao Tong University[J]. Laboratory Animal and Comparative Medicine, 2024, 44(2): 227-233. DOI: 10.12300/j.issn.1674-5817.2023.101.

图/表 3

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设备维护项目	每次	每天	每月	每季度	每半年	每年
Maintenance projects	Each time	Daily	Monthly	Per quarter	Semiannually	Per annum
初效过滤器Primary efficiency filter			②	③
中效过滤器Medium efficiency filter					③
高效过滤器High efficiency filter						③
传递窗中紫外灯Ultraviolet lamp in delivery window	①			③
生物安全柜中紫外灯Ultraviolet lamp in biosafety cabinet	①			③^*
细胞培养室中紫外灯Ultraviolet lamp in cell culture room		①			③
落下菌检测Airborne bacteria detection			④
尘埃粒子检测Dust particle detection				④

设备维护项目	每次	每天	每月	每季度	每半年	每年
Maintenance projects	Each time	Daily	Monthly	Per quarter	Semiannually	Per annum
初效过滤器Primary efficiency filter			②	③
中效过滤器Medium efficiency filter					③
高效过滤器High efficiency filter						③
传递窗中紫外灯Ultraviolet lamp in delivery window	①			③
生物安全柜中紫外灯Ultraviolet lamp in biosafety cabinet	①			③^*
细胞培养室中紫外灯Ultraviolet lamp in cell culture room		①			③
落下菌检测Airborne bacteria detection			④
尘埃粒子检测Dust particle detection				④

消毒物品或位置	每次	每天	每周	每月
Sterilized items or locations	Each time	Daily	Weekly	Monthly
实验室台面Laboratory countertop	②③	①④
生物安全柜台面Biosecurity countertop	①②③
仪器操作台面Instrument operating table	②	①④
细胞培养室Cell culture room		①④
传递窗Delivery window	①③	②
进出口缓冲间Inlet and outlet buffer		①③④
冰箱Icebox			②
二氧化碳培养箱内部及水盘Carbon dioxide incubator inside and water tray			②
置物架Storage shelf		①④	②
储物柜Locker		①④	②
门 Door		①④	②
墙壁 Wall		①④	②
天花板 Ceiling		①④		②

消毒物品或位置	每次	每天	每周	每月
Sterilized items or locations	Each time	Daily	Weekly	Monthly
实验室台面Laboratory countertop	②③	①④
生物安全柜台面Biosecurity countertop	①②③
仪器操作台面Instrument operating table	②	①④
细胞培养室Cell culture room		①④
传递窗Delivery window	①③	②
进出口缓冲间Inlet and outlet buffer		①③④
冰箱Icebox			②
二氧化碳培养箱内部及水盘Carbon dioxide incubator inside and water tray			②
置物架Storage shelf		①④	②
储物柜Locker		①④	②
门 Door		①④	②
墙壁 Wall		①④	②
天花板 Ceiling		①④		②

实验物品 Experimental item	高压灭菌 Autoclave	化学消毒		物理消毒
		Chemical disinfection		Physical disinfection
		杀孢子剂	75%乙醇溶液	紫外线+臭氧
		Sporicide	75% alcohol	UV+Ozone
防护用品Protective equipment
防护服Protective suits	√
拖鞋 Slippers		√		√
口罩Face masks^*		√		√
帽子Hats^*		√		√
手套Gloves^*		√		√
实验用品 Laboratory supplies
超纯水Ultrapure water	√
擦拭纸 Wiping paper	√
离心管Centrifuge tubes	√
细胞Cell ^*			√
细胞计数板Cell counting plates^*		√		√
细胞培养耗材Cell culture consumables^*		√		√
细胞培养试剂^Cell culture reagents^		√		√
仪器设备Instruments and equipment
移液器 Pipettors			√	√
精密小型仪器Precision small instruments			√	√
小型仪器 Small instruments		√		√
大型仪器 Large instruments		√		√