子痫前期大鼠模型建立及相关指标监测

doi:10.3969/j.issn.1674-5817.2015.06.004

实验动物与比较医学 ›› 2015, Vol. 35 ›› Issue (6): 448-452.DOI: 10.3969/j.issn.1674-5817.2015.06.004

子痫前期大鼠模型建立及相关指标监测

周晶^1,2, 严国锋¹, 罗章源³, 陈东宝¹, 陈学进¹, 李垚¹

1. 上海交通大学医学院实验动物科学部, 上海 200025;
2.复旦大学生命科学学院, 上海 200433;
3. 杰升生物科技(上海)有限公司, 上海 201112

收稿日期:2015-09-15 出版日期:2015-12-25 发布日期:2015-12-25
作者简介:周晶(1985-), 女, 生物工程硕士研究生在读。E-mail: zj2008@shsmu.edu.cn

Establishment of Preeclampsia Model in Rat and Detection on Related Indicators

ZHOU Jing^1,2, YAN Guo-feng¹, LUO Zhang-yuan³, CHEN Dong-bao¹, CHEN Xue-jin¹, LI Yao¹

1. Department of Laboratory Animal Science; Shanghai Jiao Tong University School of Medicine, Shanghai 20025, China;
2. School of Life Sciences Fudan University, Shanghai 200433, China;
3. Genix Biotek Science Technology Co.Ltd., Shanghai 201112,China

Received:2015-09-15 Published:2015-12-25 Online:2015-12-25

摘要/Abstract

摘要： 目的使用SD大鼠构建子痫前期动物模型,并采用遥测法监测模型大鼠的动脉血压,分析掌握模型动物在自由活动状态下的血压及其他各项生理生化指标。方法将16只SD大鼠分为4组, 遥测模型组A: 大鼠植入遥测子并构建子痫前期模型。模型组B: 仅构建子痫前期模型。遥测对照组C: 植入遥测子并制作模型假手术。对照组D: 仅制作子痫前期假手术。A、C组在遥测子植入后连续监测血压数据。各组收集妊娠13.5 d、20.5 d尿液检测尿总蛋白、尿肌酐。妊娠21.5 d临产前将母鼠安乐死,取肝、肾、胎盘组织进行HE染色。结果模型组A制模2 d后平均动脉压、收缩压、舒张压较对照组C显著上升(P <0.01),妊娠21.5 d时平均动脉压、收缩压、舒张压较对照组C显著降低(P <0.01)。模型组A、B尿总蛋白/肌酐比值显著高于对照组C、D (P<0.01),同时A、B两组肝、肾、胎盘组织结构也出现了改变,并且出现70.49%胎鼠发育停止。结论成功构建子痫前期大鼠模型,获得大鼠在自由活动状态下的动脉压、尿生化、胎盘胎鼠发育情况, 掌握了模型动物在妊娠期真实的、连续的血压变化周期。该模型或可成为子痫前期疾病研究的理想动物模型。

关键词: 生理遥测, 子宫胎盘缺血再灌注模型(RUPP), 子痫前期(PE), 平均动脉压(MAP)

Abstract: Objectives To establish preeclampsia SD rat model and monitor the alterations of related indicators in conscious rats. Methods Sixteen SD rats were divided into 4 groups randomly. Telemetrical modeling group A, which was implanted radiotelemetry and built preeclampsia model. Group B was modeling control group, which only built preeclampsia model without radiotelemetry implantation. Group C was implanted radiotelemetry and operated sham-operation of preeclampsia modeling. Group D was only operated sham-operation of preeclampsia modeling. Blood pressure of rats in groups A and C were monitored continuously after radiotelemetry implantation. Urine samples were collected from each group at pregnant day 13.5 and day 20.5 for urine TP and creatinine test. Pregnant rats were euthanieed at day 21.5 of pregnancy, and then the liver, kidney and placenta tissues were collected for HE staining. Results Mean arterial pressure, systolic pressure and diastolic pressure of group A were significant higher than those of group C (P<0.01) after modeling 2 days, but at the day 21.5 of pregnancy, mean arterial pressure, systolic pressure and diastolic pressure of group A were significantly decreased compared to group C (P<0.01). In groups A and B urine TP/creatinine ratio were significantly higher than those of groups C and D(P<0.01), also their liver, kidney and placenta tissue structure get pathological changes, and 70.49% fetal growth were ceased in groups A and B. Conclusion Preeclampsia model rats have been established successfully. This rat model will contribute to research of preeclampsia.

Key words: Radiotelemetry, Reduced uterine perfusion pressure (RUPP), Preeclampsia(PE), Mean arterial pressure(MAP)

中图分类号:

Q95-33

周晶,严国锋,罗章源,等. 子痫前期大鼠模型建立及相关指标监测[J]. 实验动物与比较医学, 2015, 35(6): 448-452. DOI: 10.3969/j.issn.1674-5817.2015.06.004.

ZHOU Jing,YAN Guo-feng,LUO Zhang-yuan,et al. Establishment of Preeclampsia Model in Rat and Detection on Related Indicators[J]. Laboratory Animal and Comparative Medicine, 2015, 35(6): 448-452. DOI: 10.3969/j.issn.1674-5817.2015.06.004.

参考文献

[1] Iasmina MC, Steven JW, Tracey LW, et al.Advances in the pathophysiology of pre-eclampsia and related podocyte injury[J]. Kindy Int, 2014, 86(2):275-285.
[2] 中国高血压防治指南修订委员会. 中国高血压防治指南[J]. 中华高血压杂志, 2011, 19(8):701-742.
[3] 孙倩倩, 古航. 胎盘合体滋养细胞中硫化氢对激活素影响的研究[J]. 实用妇产科杂志, 2014, 30(7):511-514.
[4] 孙怡琳. 合体滋养细胞微绒毛制备子痫前期动物模型的研究[D]. 重庆: 第三军医大学, 2013.
[5] McCarthy FP, Kindom JC, Kenny LC, et al. Animal models
of preeclampsia; uses and limitations[J]. Placenta, 2011, 32(6):413-419.
[6] Yang X, Guo L, Sun X, et al.Protective effects of hydrogenrich saline in preeclampsia rat model[J].Placenta, 2011, 32(9):681-686.
[7] Hossain N, Khan N, Shah N, et al.Spot urine protein-creatinine ratio and 24-h urine protein excretion:Diagnostic accuracy in women with pre-eclampsia[J].Pregnancy Hypertens, 2014, 4(1):87-90.
[8] 俞丽丽, 李力, 陈鸣, 等. 寒冷刺激诱发孕鼠妊娠高血压综合征动物模型研究[J]. 第三军医大学学报, 2001, 23(4):419-421.
[9] 王忠民, 王波, 叶萍, 等. 精氨酸对大鼠妊娠高征的治疗作用及其机制的探讨[J]. 上海实验动物科学, 2003, 23(3):148-150.
[10] Barbara TA, Maria TL, Walter CK, et al.L-arginine attenuates hypertension in pregnant rats with reduced uterine perfusion pressure[J]. Hypertension, 2004, 43(4):832-836.
[11] 李静, 唐卉. 低剂量内毒素诱导构建子痫前期大鼠模型[J]. 山东医药, 2014, 54(9):26-28.
[12] Ashley JB, Christopher TB, Karen N, et al.Pravastatin attenuates hypertension, oxidative stress, and angiogenic imbalance in rat model of placental ischemia-induced hypertension[J]. Hypertension, 2013, 61(5):1103-1110.
[13] Alexander BT, Kassab SE, Miller MT, et al.Ruduced uterine perfusion pressure during pregnancy in the rat is associated with increases in arterial pressure and changes in renal nitric oxide[J].Hypertension, 2001, 37(4):1191-1195.
[14] Javadian P, Salmanian B, Javadi-Paydar M, et al.Effect of morphine on the reduced uteroplacental perfusion model of pre-eclampsia in rats[J]. Eur J Obstet Gynecol Reprod Biol, 2013, 168(2):161-166.

[1]	潘林琴, 邓向亮, 罗云霞. 缺血性脑卒中动物模型的中西医整合转化研究进展[J]. 实验动物与比较医学, 2026, 46(3): 344-356.
[2]	高一龙, 贺星亮, 周肖鹏, 李大伟, 包喜军, 李来有. 全基因组重测序挖掘史宾格母犬的产仔数性状候选基因[J]. 实验动物与比较医学, 2026, 46(3): 378-387.
[3]	王娟, 徐佳慧, 田韵远, 张萌萌, 李敏, 王四旺, 李瑶. 两种雌性小鼠溃疡性结肠炎模型的比较与行为学观察[J]. 实验动物与比较医学, 2026, 46(3): 332-343.
[4]	陈兵, 谢晓婕, 陶体富, 王敬东, 邹弈星. 四川省实验动物从业人员培训教育现状及对策研究[J]. 实验动物与比较医学, 2026, 46(3): 456-463.
[5]	汤建平, 赵丽亚, 赵莹. 常用近交系大鼠微卫星遗传位点筛选及分析[J]. 实验动物与比较医学, 2026, 46(3): 388-396.
[6]	卜瑜, 候金婷, 李媛媛, 沙静涛, 解晨露, 王文刚, 孙兴伟. 中西医病证结合的痔疮动物模型研究现状与评价[J]. 实验动物与比较医学, 2026, 46(3): 357-366.
[7]	卜纪雯, 华叶, 金仕容, 任宁欣, 李福宁, 杜久林. 斑马鱼品系及斑马鱼鱼房的自动化管理[J]. 实验动物与比较医学, 2026, 46(3): 416-425.
[8]	王秀然, 李浩, 陈正涛, 于杨, 张素英, 陶如, 王可洲. 医学院校实验动物专业“三位一体”人才培养体系建设的创新与实践[J]. 实验动物与比较医学, 2026, 46(3): 446-455.
[9]	李嘉菲, 张朕豪, 王硕, 田歌, 温爽, 闫玉雪, 崔然, 叶振, 崔永春. 自主神经调节剂对房颤模型大鼠心房电重塑及组织病理变化的影响[J]. 实验动物与比较医学, 2026, 46(3): 321-331.
[10]	艾秀峰, 张利棕, 方明笋, 吕东颖, 陈楚, 蔡兆伟, 王德军. 16S rRNA测序分析饮用含氯水对大鼠和小鼠肠道菌群的影响差异[J]. 实验动物与比较医学, 2026, 46(3): 437-445.
[11]	周忆旻, 张昕宇, 杨佳诺, 刘梦佳, 司灿灿, 叶海绿, 孙文超, 兰添. MHV、MPV和Reo-3三重TaqMan定量PCR检测方法的建立[J]. 实验动物与比较医学, 2026, 46(3): 408-415.
[12]	孙强. 克隆能否让哺乳动物“永生”：20年58代小鼠给出的回答[J]. 实验动物与比较医学, 2026, 46(3): 464-466.
[13]	陈林, 张厚森, 艾曼, 漆重阳. 数字化转型赋能江苏省实验动物管理高质量发展路径研究[J]. 实验动物与比较医学, 2026, 46(3): 426-436.
[14]	李龙雪, 万崇凡, 张琦, 雷茹婷, 王潇玥, 程乐妍, 赖琦, 刘荣华, 刘漩, 徐铁龙. 基于miRNA测序分析清肺排毒汤防治小鼠急性肺损伤的分子机制研究[J]. 实验动物与比较医学, 2026, 46(3): 311-320.
[15]	王璐, 王青杰, 杨春弘, 张一鉴. 新方法学在生物医学领域的应用及其对“3R”原则的践行[J]. 实验动物与比较医学, 2026, (): 1-10.

子痫前期大鼠模型建立及相关指标监测

Establishment of Preeclampsia Model in Rat and Detection on Related Indicators

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价