肺不张后复张小型猪肺泡表面活性物质A的表达及CT比较

doi:10.3969/j.issn.1674-5817.2015.06.002

实验动物与比较医学 ›› 2015, Vol. 35 ›› Issue (6): 436-514.DOI: 10.3969/j.issn.1674-5817.2015.06.002

肺不张后复张小型猪肺泡表面活性物质A的表达及CT比较

涂军伟¹, 王赛斌¹, 盛怡俊¹, 陈慧君¹, 田江华¹, 盛琳¹, 应明亮², 胡斌³, 何忠平⁴

1. 金华市中心医院呼吸科, 金华 321000;
2. 金华市中心医院影像科, 金华 321000;
3. 金华市中心医院病理科, 金华 321000;
4. 金华市食品药品检验所, 金华 321000

收稿日期:2015-06-15 出版日期:2015-12-25 发布日期:2015-12-25
作者简介:涂军伟(1972-), 男, 硕士, 主任医师, 从事呼吸内科专业。E-mail: tujw2008@126.com
基金资助:
浙江省科技计划项目(2012C37090)

Expression of Pulmonary Surfactant-associated Protein A and CT Imaging in Lung Re-expansion Minipig after Atelectasis

TU Jun-wei¹, WANG Sai-bin¹, SHENG Yi-jun¹, CHEN Hui-jun¹, TIAN Jiang-hua¹, SHENG Lin¹, YING Ming-liang², Hu Bin³, HE Zhong-ping⁴

1. Department of Pneumology,
2. Department of Radiology,
3. Department of Pathology, Jinhua Municipal Central Hospital, Jinhua 321000, China;
4. Jinhua Municipal Institute for Food and Drug Control, Jinhua 321000, China

Received:2015-06-15 Published:2015-12-25 Online:2015-12-25

摘要/Abstract

摘要： 目的探讨肺不张后复张过程中小型猪肺泡表面活性物质A(SPA)的表达及多层螺旋计算机断层扫描(CT)影像学变化。方法采用18只中国实验用小型猪I系为实验动物,在支气管镜下置入封堵器造成肺不张模型,分别在肺不张后3 d、1周及2周取出封堵器,运用CT观察各组肺复张情况,在取出封堵器后2周取实验肺组织,采用免疫组织化学检测SPA的表达。结果 SPA在肺不张2周组表达下降,与3 d组、1周组比较存在统计学差异(P<0.05)。CT下比较3组单侧肺不张面积无统计学差异(P>0.05),而在复张过程中,3 d组残留肺不张面积最少,与1周组、2周组比较存在统计学差异( P<0.05)。取出封堵器后,肺不张1周、2周组之间CT弧度值无明显差异(P>0.05),但均高于3 d组(P<0.05)。结论肺复张后SPA表达随肺不张时间延长而减少;肺不张后肺复张CT影像学改善取决于发生肺不张时间,此时间在一周内为佳。

关键词: 肺不张, 复张, 肺泡表面活性物质A(SPA), 计算机断层扫描(CT), 弧度值

Abstract: Objectives To investigate the expression of Pulmonary surfactant-associated protein A(SPA) and the CT imaging in lung re-expansion after atelectasis. Methods The minipig model of lung re-expansion after atelectasis was established. Male China Experimental Miniature Pig-I were randomly divided into three-days atelectasis group (3 d), one-week atelectasis group (1 week) and two-weeks atelectasis group (2 weeks), respectively (n=6). Pigs were euthanized after two weeks of lung re-expansion. The expression of SPA of experimental lung tissue was detected by immunohistochemistry. At specified time point, CT value and imaging changes were measured. Results The expression of SPA was lower in 2 weeks group than that in the other two groups (P<0.01). The CT value was lower in 3 d group than that in the other two groups (P<0.01), and the degree of lung re-expansion was the highest level in 3 d group among three groups. Conclusions The SPA expression of experimental lung tissue was decreased with atelectasis prolonged. The CT imaging indicated that improvement of lung re-expansion was dependent on the time of the atelectasis and the preferable opportunity of atelectasis cure was within one week.

Key words: Atelectasis, Re-expansion, Pulmonary surfactant-associated protein A(SPA), CT value

中图分类号:

Q95-33

涂军伟,王赛斌,盛怡俊,等. 肺不张后复张小型猪肺泡表面活性物质A的表达及CT比较[J]. 实验动物与比较医学, 2015, 35(6): 436-514. DOI: 10.3969/j.issn.1674-5817.2015.06.002.

TU Jun-wei,WANG Sai-bin,SHENG Yi-jun,et al. Expression of Pulmonary Surfactant-associated Protein A and CT Imaging in Lung Re-expansion Minipig after Atelectasis[J]. Laboratory Animal and Comparative Medicine, 2015, 35(6): 436-514. DOI: 10.3969/j.issn.1674-5817.2015.06.002.

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肺不张后复张小型猪肺泡表面活性物质A的表达及CT比较

Expression of Pulmonary Surfactant-associated Protein A and CT Imaging in Lung Re-expansion Minipig after Atelectasis

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