一株鸭源新城疫病毒分子特性及对SPF鸡、鸭致病性的比较研究

doi:10.3969/j.issn.1674-5817.2015.01.014

实验动物与比较医学 ›› 2015, Vol. 35 ›› Issue (1): 65-70.DOI: 10.3969/j.issn.1674-5817.2015.01.014

一株鸭源新城疫病毒分子特性及对SPF鸡、鸭致病性的比较研究

吴伟¹, 刘怀然², 刘胜旺^1,2, 陈洪岩^1,2

1.东北农业大学, 哈尔滨 150030;
2.中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室, 哈尔滨150001

收稿日期:2014-12-08 出版日期:2015-02-25 发布日期:2015-02-25
作者简介:吴伟(1988-), 男, 硕士研究生。 E-mail:709558484@qq.com
基金资助:
公益性行业(农业)科研专项经费项目(201303033)

Molecular Characteristics and Pathogenicity of Newcastle Disease Virus from Domestic Ducks in China

WU Wei¹, LIU Huai-ran², LIU Sheng-wang^1,2, CHEN Hong-yan^1,2

1. Northeast Agricultural University, Harbin 150030, China;
2. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China

Received:2014-12-08 Online:2015-02-25 Published:2015-02-25

摘要/Abstract

摘要： 目的研究一株鸭源新城疫病毒分子的特性,并比较其对SPF鸡和SPF鸭致病性的不同。方法将分离到的病毒克隆、测序并用SPF鸡和SPF鸭分别评价该毒株的致病性。结果 Du/CH/LGD/358/2011的基因组由15 198个核苷酸序列组成,编码6种结构蛋白。F基因裂解位点的氨基酸序列为112 ERQER/L117。通过全基因组核苷酸序列和F基因高变区核苷酸序列构建遗传进化树,证明该毒株属于class I基因3b型,与香港活禽市场分离到的毒株类似,但略有不同。该毒株对SPF鸡和SPF鸭的致病性基本一致。结论该毒株为弱毒株,使用SPF鸡和SPF鸭均可以对鸭源弱毒株进行致病性评价,为SPF鸭的检测提供理论依据。

关键词: 新城疫病毒, SPF鸭, SPF鸡, 致病性, 分子特性

Abstract: Objective To study the molecular characteristics of Newcastle disease virus from domestic ducks, and then compare the pathogenicity in SPF chicken and ducks. Methods By cloning and sequencing the complete genomic sequence of Newcastle disease virus (NDV) strain Du/CH/LGD/358/2011, and then conducted the pathogenicity test in SPF chicken and ducks. Results The genome was 15 198 nucleotides (nt) in length, followed the “rule of six” that is required for replication of NDV strains, and encoded six structural polypeptides. The cleavage motif of F protein was 112 ERQER/L117, which was the characteristic of lentogenic strain. Phylogenetic analysis based on complete genomic sequences and partial sequences of F gene revealed that Du/CH/LGD/358/2011 belonged to class I genotype 3b, an independent clade closely related to Hong Kong LBM viruses. In addition, the pathogenic results between SPF chickens and SPF ducks was hardly varied. Conclusion Du/CH/LGD/358/2011 belonged to lentogenic strain, both SPF chicken and ducks could estimating be used fpr the pathogenicity. This study provided the theoretical basis for mornitoring on SPF ducks.

Key words: Newcastle disease virus, SPF duck, SPF chicken, Pathogenicity, Molecular characteristics

中图分类号:

Q95-33

吴伟, 刘怀然, 刘胜旺, 陈洪岩. 一株鸭源新城疫病毒分子特性及对SPF鸡、鸭致病性的比较研究[J]. 实验动物与比较医学, 2015, 35(1): 65-70.

WU Wei, LIU Huai-ran, LIU Sheng-wang, CHEN Hong-yan. Molecular Characteristics and Pathogenicity of Newcastle Disease Virus from Domestic Ducks in China[J]. Laboratory Animal and Comparative Medicine, 2015, 35(1): 65-70.

参考文献

[1] 孙委委, 孙初阳, 张晓楠, 等. 一株基因Ⅱ 型新城疫病毒的分离及基因组分析[J]. 中国预防兽医学报, 2010 (3):175-178.
[2] de Leeuw O, Peeters B. Complete nucleotide sequence of Newcastle disease virus: evidence for the existence of a new genus within the subfamily Paramyxovirinae[J]. J Gen Virol, 1999, 80(1):131-136.
[3] Steward M, Vipond IB, Millar NS, et al.RNA editing in Newcastle disease virus[J]. J Gen Virol, 1993, 74(12):2539-2548.
[4] Kim LM, King DJ, Curry PE, et al.Phylogenetic diversity among low-virulence Newcastle disease viruses from waterfowl and shorebirds and comparison of genotype distributions to those of poultry-origin isolates[J]. J Virol, 2007, 81(22):12641-12653.
[5] Kaleta EF, Baldauf C.Newcastle disease in free-living and pet birds[M]. In: Alexander DJ(ed). Newcastle disease. New York: Springer , 1988: 197-246.
[6] Shengqing Y, Kishida N, Ito H, et al.Generation of velogenic Newcastle disease viruses from a nonpathogenic waterfowl isolate by passaging in chickens[J]. Virology, 2002, 301(2): 206-211.
[7] Peeters BPH, Gruijthuijsen YK, De Leeuw OS, et al.Genome replication of Newcastle disease virus: involvement of the rule-of-six[J]. Arch Virol, 2000, 145(9):1829-1845.
[8] 石跃, 刘怀然, 刘培欣, 等. 一株野鸭源基因 Ⅲ 型新城疫病毒主要生物学特性及基因组序列测定分析[J]. 中国预防兽医学报, 2011, 33(5):391-394.
[9] Kim LM, King DJ, Curry PE, et al.Phylogenetic diversity among low-virulence Newcastle disease viruses from waterfowl and shorebirds and comparison of genotype distributions to those of poultry-origin isolates[J]. J Virol, 2007, 81(22):12641-12653.
[10] Kim LM, King DJ, Suarez DL, et al.Characterization of class I Newcastle disease virus isolates from Hong Kong live bird markets and detection using real-time reverse transcription-PCR[J]. J Clin Microbiol, 2007, 45(4):1310-1314.

[1]	于海波, 周洁, 李剑虹, 李昌文, 高彩霞, 夏长友. SPF鸡福利伦理指南团体标准的制定和解读[J]. 实验动物与比较医学, 2022, 42(6): 572-576.
[2]	周妍, 刁晨曦, 张圆圆, 于海波, 陆涛峰, 赵丽丽, 陈洪岩. SPF鸡不同生长阶段粪便菌群组成及多样性研究[J]. 实验动物与比较医学, 2017, 37(3): 231-239.
[3]	梁殊林, 李慧昕, 陈洪岩, 刘胜旺. 表达新城疫病毒血凝素神经氨酸酶(HN)基因重组鸭肠炎病毒的构建[J]. 实验动物与比较医学, 2015, 35(3): 195-201.
[4]	李新华, 张世栋, 姜亦飞, 王春玲, 李峰. SPF鸡舍建设工程设计与工艺[J]. 实验动物与比较医学, 2014, 34(4): 339-342.
[5]	杨金兴, 王友令, 欧阳文军, 袁小远. 提高SPF鸡蛋合格率的综合措施[J]. 实验动物与比较医学, 2013, 33(2): 157-159.
[6]	欧阳文军, 王友令, 杨金兴, 袁小远, 孙晓军. SPF鸡饲养管理制度的建立[J]. 实验动物与比较医学, 2012, 32(4): 353-355.
[7]	王佳乐, 张江, 张瑞忠. SPF鸡在隔离器内中早期生长模型的拟合探讨[J]. 实验动物与比较医学, 2012, 32(1): 70-72.
[8]	窦如海1，简政1，王应文1，李浩2，郑茂恩2，朱瑞良3. SPF鸡(蛋)质量控制相关程序的建立与完善[J]. , 2010, 30(6): 455-461.
[9]	金东庆1，徐凯勇2，单忠芳3，朱果4，窦如海1，冯墨竹1. 国内与山东SPF鸡(蛋)生产现状、存在问题与对策[J]. , 2008, 28(5): 345-348.
[10]	刘忠华, 王颖彦, 黄韧, 刘香梅, 邓少嫦. 禽流感H5N1型病毒对沙鼠致病性的初步研究[J]. , 2005, 25(4): 199-202.
[11]	厉从志, 梁洪宇. 辐照饲料在SPF鸡饲养中的使用结果[J]. , 2000, 20(2): 118-119.
[12]	薛正风, 李厚达, 金韶华, 郭朝康. 洁净垂直层流架饲养SPF鸡的研究[J]. 实验动物与比较医学, 1994, 14(3,4): 151-154.

一株鸭源新城疫病毒分子特性及对SPF鸡、鸭致病性的比较研究

Molecular Characteristics and Pathogenicity of Newcastle Disease Virus from Domestic Ducks in China

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 12

编辑推荐

Metrics

本文评价