束缚应激致亚健康状态大鼠的免疫功能改变与中药干预的影响

›› 2010, Vol. 30 ›› Issue (2): 117-120.

束缚应激致亚健康状态大鼠的免疫功能改变与中药干预的影响

浙江中医药大学动物实验研究中心/比较医学研究中心，杭州 310053

收稿日期:2009-12-08 出版日期:2010-02-28 发布日期:2010-02-28
基金资助:
1.浙江省中医“治未病”研究专项（2007WB022）；2.受“浙江省卫生高层次创新人才培养工程项目”资助。

Immune function of Sub-healthy Rats Induced by Restraint Stress and Altered by Traditional Chinese Medicine

Zhejiang Chinese Medical University Laboratory Animal Research Center, Hangzhou 310053,China

Received:2009-12-08 Online:2010-02-28 Published:2010-02-28

摘要/Abstract

摘要： 目的观察束缚应激致亚健康状态大鼠模型的免疫功能的改变，探讨中药的干预作用及其机制.方法 40只雄性SD大鼠，随机分为正常对照组、模型对照组、抗衰老片组和六味地黄丸组，采用限制活动模拟人类心理应激因素，建立大鼠亚健康状态模型，同时，用抗衰老片和六味地黄丸进行干预，测定应激大鼠的免疫功能指标.结果造模21 d后，模型大鼠的T淋巴细胞增殖能力、NK细胞活性、IL-2和IFN-γ含量及胸腺指数均显著降低(P＜0.05).给予抗衰老片和六味地黄丸后，模型大鼠T、B淋巴细胞增殖能力、胸腺指数，IL-2含量均显著升高(P＜0.05)，NK细胞活性有升高趋势.结论束缚应激致亚健康状态大鼠模型的免疫功能下降，给予抗衰老片和六味地黄丸后，可明显提高亚健康状态大鼠的免疫功能，提示中药提高免疫功能的作用可能与调节机体细胞与体液免疫有关.

关键词: 束缚应激, 亚健康, 免疫功能, 中药

Abstract: Objective To observe the changes of immune function in sub-health state rat model induced by restraint stress and to explore the mechanism of the traditional Chinese medicine alters the stress . Methods Forty Male SD rats were randomly divided into 4 groups: normal control group，model control group, anti-ageing tablets group and Liuwei Dihuang pills group. To simulate human physiological stress, the sub-health state rat model was induced by restraint . The rats in administration group were given anti-aging tablets and Liuwei Dihuang pills respectively during the model establishment process. At the end of experiment, the indexes of immune function of sub-health state rats tested.Results Twenty-one days afler the experiment, T lymphocyte proliferation capacity, NK cell activity,content of serum IL-2, IFN-γ and thymus index decreased significantly (P<0.05). After treatment of anti-aging tablets and Liuwei Dihuang pills，T and B lymphocyte proliferation capacity，thymus index and content of serum IL-2 were significantly increased (P<0.05), NK cell activity had an increasing trend.Conclusion The immune function decreased in sub-health state rat model induced by restraint stress，and anti-ageing tablets and Liuwei Dihuang pills could improve the immune function of sub-health state rats significantly.The result suggested that the effect of improving immune function by traditional Chinese medicine may be related to regulate cellular and humoral immunity.

Key words: Restraint stress, Sub-health state rats, Immune function;Traditional Chinese medicine (TCM)

艾秀峰，冷晓霞，陈民利，朱科燕，周卫民，陶涛，潘永明，屠珏. 束缚应激致亚健康状态大鼠的免疫功能改变与中药干预的影响[J]. , 2010, 30(2): 117-120.

AI Xiu-feng,LENG Xiao-xia,CHEN Min-li,ZHU Ke-yan,ZHOU Wei-min,TAO Tao,PAN Yong-ming,TU Jue. Immune function of Sub-healthy Rats Induced by Restraint Stress and Altered by Traditional Chinese Medicine[J]. , 2010, 30(2): 117-120.

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