›› 2008, Vol. 28 ›› Issue (4): 225-229.

所属专题: 实验动物资源开发与利用

• 论文 • 上一篇    下一篇

皖南山区猕猴种群的形态特征与微卫星遗传多样性初步分析

  

  1. 1.安徽大学生命科学学院,安徽省生态工程与生物技术重点实验室,合肥 230039;2.安徽省生态经济技术研究中心,合肥 230039;3.安徽省体育科学技术研究所,合肥 230001;4.安徽省实验猕猴中心,黄山 245604
  • 收稿日期:2008-04-01 出版日期:2008-04-30 发布日期:2008-04-30
  • 基金资助:
    国家自然科学基金(30570237);安徽省教育厅科技创新团队计划项目(TD200703);安徽省优秀青年基金(040443070);安徽省教育厅自然科学基金(2005KJ02IZD),安徽省人才基金;安徽省科技厅科技条件专项,安徽大学学术创新团队项目资助

Morphological Characters and Genetic Polymorphism Analysis by Microsatellite Loci in Rhesus Monkey Stock from Wannan Mountains

  1. 1. School of Life Science, Anhui University,Anhui Key Laboratory of Ecological Engineering and Biotechnology, Hefei 230039, China;2. Ecological Economy Technology Research Center of Anhui Province, Hefei 230039, China;3. Anhui Institute of Sport Science and Technology, Hefei 230001, China;4. Laboratory Rhesus Monkey Center of Anhui Province, Huangshan 245604, China
  • Received:2008-04-01 Online:2008-04-30 Published:2008-04-30

摘要: 目的 为建立封闭群实验猕猴,对皖南山区猕猴种群的遗传背景进行了初步调查.方法 活体测量了18只猕猴的形态特征变量,并使用微卫星标记对33只猕猴进行了遗传多样性分析.结果 体重变量具有显著的性别差异,躯干变量具有显著的年龄差异.同时从12个微卫星标记中筛选出9个具多态性标记,在猕猴群中平均检测到8个等位基因,群体杂合度H=0.825±0.106,多态信息含量PIC=0.788±0.121.结论 皖南山区猕猴种群的形态特征体现了福建亚种的特性,在微卫星水平上表现出较高的遗传多样性,适合进行引种并建立猕猴的封闭群.

关键词: 猕猴种群, 形态特征, 微卫星位点, 遗传多样性

Abstract: Objective In order to establish the closed colony, the heredity background of the rhesus monkeys from Anhui stock were investigated. Method Several morphological variables of 18 individual monkeys from Anhui Wannan Mountains were measured, and the genetic polymorphism of 33 monkeys was analyzed by micro satellite loci. Result The results of f-test showed that there were evident differences in weight between male and female and that in trunk length between adult and subadult. At the same time, nine polymorphic microsatellite loci were selected from twelve loci. The mean number of alleles of Anhui rhesus monkey stock detected was 8. The heterozygosity value was 0.825±0.106, and the polymorphism information content value was 0.788±0.121. Conclusion Morphological traits of Anhui rhesus monkey stock embodied the feature of M.mulatta littoralis. And genetic diversity of the group is high on the microsatellite level. So we can screen out the original seed from Anhui rhesus monkey stock and establish the closed colony.

Key words: Anhui rhesus monkey stock, Morphological character, Micorsatellite locus;Genetic polymorphism