ZHOU Jia-liang, HU Ying, SHA Xiao-min, YANG Fei, AI Xiao-jie
Objective To study the impact of frequently-used material intervention measures towards stress-related index of laboratory animals. Methods Sixty weaning male KM mice were randomly assigned to five groups: A was routine feeding group, B nutritional intervention group, C sunflower seed group, D toy group, and E incense group, with 12 mice in each group. Food and water intake were measured every day. Body weight was measured every two days. Blood sample was collected 37 days later from the caudal vein in mouse tail and blood routine examination has been performed. mRNA index of GR, POMC, nNOS, BDNF, NMDA, TH and TPH in brain tissues of the mice were measured by fluorescent real-time PCR method and differences between material intervention groups and routine feeding group were compared. Results Compared with group A, the body weights of mice in other groups reduced. For food intake, group B reduced compared with group A, while group D and E increased. For water intake, group B reduced compared wtih group A, while group D and E increased. For the level of BDNF mRNA expression, comparing with group A, group F and E increased obviously, while group C reduced to some extent. For the level of GR mRNA expression, comparing with group A, group D reduced obviously, while group C and E reduced to some extent. For the level of POMC mRNA expression, comparing with group A, group D reduced to some extent, while other groups reduced significantly. For the level of NMDA mRNA expression, comparing with group A, group D increased obviously. For the level of TH mRNA expression, comparing with group A, group B and C reduced to some extent. For the level of TPH mRNA expression, comparing with group A, group C reduced to some extent. Hematological parameters had no obvious difference among all groups. Conclusions Placing toys makes food and water intake rise sharply, ability to resist fear and anxiety enhanced obviously, learning and memory ability reinforced greatly and stress-related index reduced evidently. Incense makes food and water intake go up, ability to resist fear and anxiety enhanced obviously and stress-related index reduced evidently .
