Advances on in Vitro Culture of Female Reproductive and Development Toxicity Based on Animal Welfare

doi:10.3969/j.issn.1674-5817.2019.02.016

Abstract

Abstract: The aim of reproductive and developmental toxicity study is to identify the effects of exogenous substances on reproductive function and development of mammals by animal experiments, predicting the potential adverse effects on reproductive cells, pregnancy, pregnancy, delivery, lactation and other parental reproductive functions, as well as on the development of offspring embryos/fetuses and postnatal development. The methods of studying the reproductive and developmental toxicity of whole animal in females have been widely used in the evaluation of reproductive and developmental toxicity of drugs, food, chemicals and cosmetics. However, its main problems are insensitivity, long cycle, and large sample size. Especially, as the reproductive function of in vivo experiments is mainly based on parameters related to reproductive and developmental consequences, it is difficult to reveal the toxic action site and mechanism. Alternative method of female reproductive and developmental toxicology refers to any method or procedure which can replace animal experiments whenever possible, reduce the required number of animals or optimize and reduce animal suffering in animal experimental program, especially in preliminary evaluation of exogenous toxic substances as a powerful complement for animal experiments. In this paper, the technique and research progress of in vitro culture of female reproductive developmental toxicity were summarized in this paper, the advances in vitro culture of female reproductive toxicity were summarized from cells and tissue culture in female reproductive system, embryonic cells and tissue culture, breast cell culture so as to provide references for the study of in vitro culture of reproductive and developmental toxicity in females.

Key words: Female, Reproductive and development toxicity, Animal welfare, In vitro culture

CLC Number:

Q95-33

LU Qin, ZHOU Yue-hua, TANG Hai-fei, WANG Zhi-wen, ZHANG Ting. Advances on in Vitro Culture of Female Reproductive and Development Toxicity Based on Animal Welfare[J]. Laboratory Animal and Comparative Medicine, 2019, 39(2): 155-162.

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